Toxicity of Uranium and Lead to B.subtilis

liuqing; Xu weichang; Zhao guodong; Zhao youcai

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Toxicity of Uranium and Lead to B.subtilis

liuqing 1 * #,Xu weichang 2,Zhao guodong 2,Zhao youcai 3

1.The State Key Laboratory of Pollution Control and Resource Reuse, Tongji University

2. School of Architectural Engineering, Resources and Environment, Nanhua University

3.The State Key Laboratory of Pollution Control and Resource Reuse, Tongji University

*Correspondence author

#Submitted by

Subject:

Funding: none

Opened online: 5 April 2007

Accepted by: none

Citation: liuqing,Xu weichang,Zhao guodong.Toxicity of Uranium and Lead to B.subtilis[OL]. [ 5 April 2007] http://en.paper.edu.cn/en_releasepaper/content/11986

B.subtilis has the broad foreground in dealing with the heavy metals waste water. It is necessary to study the toxicity of heavy metals to B.subtilis and get “the Maximum No-Effect Level ” and “the Lethal Concentration”.The toxicity of different concentration Uranium and Lead to B.subtilis in its growth was studied by three different methods in terms of measuring the diameter of the circle of bacteriostasis, counting the bacterial population by Heamacytometer and observing the change of the shape and the density of the bacteria through microscope. The experimental results have indicated that these two heavy metals had great inhibition effect to the growth of B.subtilis. The Maximum No-Effect Level of Uranium and Lead to B.subtilis are below 1mg/L and the Lethal Concentration of Uranium and Lead to B.subtilis are 500mg/L and 1000mg/L respectively. In these methods used for the toxicity of heavy metals to microorganism，counting the bacterial population are more sensitive.

Keywords:toxicity；B.subtilis；Uranium；Lead

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