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The effects of Mg2+, Mn2+ or Zn2+ addition on gamma-CGTase production by Bacillus macorous WSH02-06 were studied. The gamma-CGTase yield was increased maximally almost three-times by the addition of Mg2+, Mn2+ and Zn2+. The addition of these cations also stimulated cell growth. The consumption of Mg2+ was promoted in the cultures with the co-existance of Mg2+ with Mn2+ or/and Zn2+. The maximal gamma-CGTase yield and dry cell weight showed a linear increasing relation with the consumption of Mg2+. The increased intracellular concentration of ATP and ATP-to-ADP ratios by the addition of these cations can provide more energy for the better cell growth and higher gamma-CGTTase production. |
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Keywords:gamma-CGTase, Bacillus macorous, yield, metal ion, consumption, ATP/ADP |
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