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Core binding factor α1 (Cbfa1) is a runt domain transcription factor that is essential for bone formation and tooth development. It is hypothesized that forced expression of Cbfa1 in dental follicle cells (DFCs) can stimulate DFCs differentiation into cementoblast-like cells in vitro. In this study we investigated the function of Cbfa1 on DFCs by retroviral system, real-time RT-PCR, Western blot and mineralization assay. Real-time PT-PCR analysis indicated that cementoblast-related genes were expressed in DFCs, including osteopontin, type I collagen, bone sialoprotein, osteocalcin and cementum attachment protein, cementum-derived protein. Overexpression of Cbfa1 in DFCs enhanced the expression of these genes. Mineralization assay also showed that Cbfa1 increased the mineralization nodules formation. The results suggest that Cbfa1 plays an important role in the differentiation of DFCs into cementoblast-like cells by increasing the expression of cementoblast-related genes.
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Keywords:Cbfa1, dental follicle cells, differentiation, overexpression, cementoblasts. |
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