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| Objective: The present study was undertaken to evaluate anti-proliferative and pro-Apoptotic activities of allicin—a plant derived organics sulfur compounds (OSC) in murine T-lymphocytes (EL-4) and try to determine its molecular mechanism of inducing apoptosis. Methods: Trypan blue dye exclusion method was used to determine cells concentration, the apoptotic rate and morphologic alteration of nuclei were measured by Hoechst 33258 fluorescent dye. DNA spallation was determined by using TUNEL kit. Protein expression of Bax, Bcl-2, cythrome C, Caspase-3, -12 were detected by Immunohistochemistry (IHC). Fluorescent microscope was used to determine the change of mitchrodria membrane potential (MMP). Result: Allicin is effective in inhibiting the proliferation of EL-4 cells in vitro in concentration-dependent manner, induces the formation of apoptotic bodies, nuclear condendensation, DNA spallation, and it even activates the expression of caspase-3, -12, cytochrome C, upregulates the ratio of the heterodimer Bax-Bcl-2 and induces MMP decrease. Conclusions: Allicin may induce apioptosis in EL-4 cells with a time- and concentration-dependent manner, in which the mitochnodria pathway may play a central role. |
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| Keywords:allicin, EL4 cell, anti-tumor, apoptosis, MMP |
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