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| Objectives: BMP-2, one of the most widely studied factors has shown some shortcomings as a therapeutic agent to enhance periodontal regeneration, while Cbfa1 is the essential transcription factor for bone formation and tooth morphogenesis. The aim of this study was to compare the function of Cbfa1 and BMP-2 on osteoblast/cementoblast related genes expression, so as to find which was more useful for periodontal regeneration.
Materials and Methods: Cbfa1 and BMP-2 recombinant retroviral plasmids were transfected into packaging cells PT67 to produce retrovirus. Then the virus was added to infect NIH3T3 and dental follicle cells (DFCs) and chosen by G418 to gain the stably overexpressed Cbfa1 or BMP-2 cells. Real-time RT-PCR, western-blotting and mineralization assay were adopted to determine and compare the effect of overexpression of Cbfa1 and BMP-2 in NIH3T3 and DFCs.
Results: We successfully obtained the cells that stably overexpressed Cbfa1 or BMP-2 by retrovirus infection. In NIH3T3, both Cbfa1 and BMP-2 induced osteoblast/cementoblast related genes expression and mineral nodules formation, but Cbfa1 increased more. In DFCs, overexpression of Cbfa1 also increased osteoblast/cementoblast related genes expression and mineral nodules formation, while overexpression of BMP-2 did not seem to effect the genes expression and mineralization.
Conclusions: By retroviral gene transfer approach, overexpression of Cbfa1 seems more useful for periodontal regeneration than that of BMP-2 through enhancing osteoblast/cementoblast related genes expression in vitro.
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| Keywords:BMP-2, Cbfa1, osteoblast/cementoblast related genes, dental follicle cells, overexpression. |
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