Cloning and Transcriptional Activity of a Novel Ovarian- Specific Promoter from Rat Retrovirus-like Elements

Chunhua Tu; Yaqin Mo; Weipeng Liu; Lili Huang; Dongzi Yang

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Cloning and Transcriptional Activity of a Novel Ovarian- Specific Promoter from Rat Retrovirus-like Elements

Chunhua Tu 1,Yaqin Mo 1 #,Weipeng Liu 2,Lili Huang 1,Dongzi Yang 1 *

1.Department of Obstetrics & Gynecology, The Second Affiliated Hospital of Sun Yat-Sen University

2.Department of Urology, The Third affiliated hospital of Sun Yat-Sen University

*Correspondence author

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Funding: 教育部博士点基金,教育部博士点新教师基金（No.20050558093,20070558250）

Opened online: 9 January 2009

Accepted by: none

Citation: Chunhua Tu,Yaqin Mo,Weipeng Liu.Cloning and Transcriptional Activity of a Novel Ovarian- Specific Promoter from Rat Retrovirus-like Elements [OL]. [ 9 January 2009] http://en.paper.edu.cn/en_releasepaper/content/27568

Tissue-specific promoters were genomic DNA sequences which controlled gene expression in a tissue-dependent manner and/or according to the developmental stage of the organism. The genes driven by these types of promoters would only be expressed in tissues where the gene products were desired, leaving the rest of tissues unmodified. Several promoters have been reported that could specifically control gene expression in an ovarian manner. However, most of them had shortage in research and clinical application. Recently, a family of ovarian-specific transcription units (OSTUs), which was specifically expressed in rat ovary, was identified with a probe generated from the long terminal repeat (LTR) of Moloney murine leukemia virus (MoMLV). In this study, we extracted a retrovirus-like sequence using RT-PCR according to the nucleotide sequences of these transcripts and proved it to be a novel ovarian-specific promoter by SYBR green real-time RT-PCR and transfecting reconstructed vectors, in which enhanced green fluorescence protein gene was under the control of this specific promoter, into ovarian and non-ovarian cell lines.

Keywords:long terminal repeat; tissue specific promoter; ovary; rat

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