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In vitro cyto-biocompability and cell detachment of temperature-sensitive dextran hydrogel
Fei Xiao 1 #,Li Chen 1 *,Rui-Feng Xing 1,Yi-Ping Zhao 1,Jing Dong 1,Gang Guo 2,Rui Zhang 2
1.Tianjin Key Laboratory of Fiber Modification and Functional Fiber
2.Tianjin Medical University
*Correspondence author
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Funding: 国家自然科学基金,教育部博士点基金(No.20574051,20050058001)
Opened online:13 January 2009
Accepted by: none
Citation: Fei Xiao,Li Chen,Rui-Feng Xing.In vitro cyto-biocompability and cell detachment of temperature-sensitive dextran hydrogel[OL]. [13 January 2009] http://en.paper.edu.cn/en_releasepaper/content/27755
 
 
A series of poly(NIPAAm-co-GMA-Dex) hydrogels were synthesized from glycidyl methacrylate-derivatized dextran (GMA-Dex) and N-isopropylacrylamide (NIPAAm) in aqueous solution. The swelling properties, cell culture and detachment behaviors of these hydrogels were investigated. It has found that poly(NIPAAm-co-GMA-Dex) hydrogels are not only sensitive to temperature in deionized water and Dulbecco’s modified eagle medium (DMEM) (10%FBS), but also exhibit good biocompatibility. In addition, L929 cells were found to detach from the hydrogel surface naturally without affecting the function of L929 by controlling environmental temperature.
Keywords:Hydrogels;N-isopropylacrylamide;Dextran;Temperature-sensitivity;Cell culture
 
 
 

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