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Magnetic Nanoparticle of Fe3O4 and 5-Bromotetrandrin interact synergistically to induce apoptosis by Daunorubicin in chronic myeloid leukemia cells
Baoan Chen 1,Jian Cheng 2,Mingfang Shen 2 * #,Feng Gao 3,Wenlin Xu 4,Jiahua Ding 2,Chong Gao 2,Qian Sun 2,Xinchen Sun 5,Guohong Li 2,Wenji Chen 2,Ningna Chen 2,Lijie Liu 6,Xiaomao Li 7,Xuemei Wang 8
1.Department of Hematology, The affiliated Zhongda Hospital
2.Department of Hematology,Zhongda Hospital
3.Department of Hematology, Zhongda Hospital
4.Department of Hematology, People’s Hospital
5.Department of Oncology, Zhongda Hospital
6.Institution of Physiology, Southeast University
7.Department of Physics, University of Saarland
8.National Key Lab of Bioelectronics(Chien-Shiung Wu Laboratory), Southeast University
*Correspondence author
#Submitted by
Subject:
Funding: 国家自然基金,国家自然基金,高等学校博士学科点专项科研基金,国家863计划(No.30740062,30872970,20070286059,2007AA0222006)
Opened online: 5 February 2009
Accepted by: none
Citation: Baoan Chen,Jian Cheng,Mingfang Shen.Magnetic Nanoparticle of Fe3O4 and 5-Bromotetrandrin interact synergistically to induce apoptosis by Daunorubicin in chronic myeloid leukemia cells [OL]. [ 5 February 2009] http://en.paper.edu.cn/en_releasepaper/content/28446
 
 
Apoptosis is a common pathway that finally mediated the killing functions of anticancer drugs, which is an important cause of MDR. The aim of this study was to investigate the potential benefit of combination therapy with MNP(Fe3O4) and BrTet. Analysis of the apoptosis percentage showed that combination of DNR with either MNP(Fe3O4) or BrTet exerted a potent cytotoxic effect on K562/A02 cells, while MNP(Fe3O4) and BrTet cotreatment can synergistically enhance DNR-induced apoptosis. Importantly, we confirmed that the distinct synergism effect of that composite on reverse multidrug resistant may owe to the regulation of various proliferative and antiapoptotic gene products, including P53 and casepase-3. Thus our in vitro data strongly suggest a potential clinical application of MNP(Fe3O4) and BrTet combination on CML.
Keywords:K562/A02 leukemic cells ;multidrug resistance; Magnetic Nanoparticle of Fe3O4;5-Bromotetrandrine; apoptosis; P53; casepase-3
 
 
 

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