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| The Drosophila extramacrochaetae protein is one of the Helix-loop-Helix superfamily proteins, which have an effect on the expression of many genes during Drosophila development. We expressed the Bombyx mori extramacrochaetae gene (Bmemc) in E. coli BL21. After purification with affinity chromatography, the fusion protein was used to prepare the polyclonal antibody. We have detected the expression of Bmemc in mRNA and protein levels. The results showed that the expression levels was highest in the tissues of testis, followed by the ovary, head, midgut and trachea; the protein was barely detectable in the Malpighian tuble, silk gland and fat body. Bmemc is highly expressed in moth, fifth instar larvae and pupae, but lower in egg. Based on the result of Notch signaling assay, we proposed that Bmemc gene may be regulated by Notch signaling pathway. |
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| Keywords:extramacrochaetae;Notch signaling pathway;real-time PCR;slikworm;γ-secretase inhibitor |
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