New amperometric glucose biosensor by entrapping glucose oxidase into chitosan/nanoporous ZrO2/multiwalled carbon nanotubes nanocomposite film

Xiurong Zhai; Jinxiang Zeng; Yanping Gao; Shuguo Gong; Wanzhi Wei

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New amperometric glucose biosensor by entrapping glucose oxidase into chitosan/nanoporous ZrO2/multiwalled carbon nanotubes nanocomposite film

Xiurong Zhai 1,Jinxiang Zeng 1,Yanping Gao 2,Shuguo Gong 2,Wanzhi Wei 2

1. College of Chemistry and Chemical Engineering, Hunan University

2.College of Chemistry and Chemical Engineering-Hunan University

*Correspondence author

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Funding: 教育部博士点基金,教育部科学技术研究重点项目（No.20020532007，03123）

Opened online:30 November 2005

Accepted by: none

Citation: Xiurong Zhai,Jinxiang Zeng,Yanping Gao .New amperometric glucose biosensor by entrapping glucose oxidase into chitosan/nanoporous ZrO2/multiwalled carbon nanotubes nanocomposite film [OL]. [30 November 2005] http://en.paper.edu.cn/en_releasepaper/content/3934

A new nanocomposite material for construction of glucose biosensor was proposed. The biosensor was formed by entrapping glucose oxidase in chitosan (CHIT)/nanoporousZrO2/multiwalled carbon nanotubes (MWNTs) nanocomposite. In this biosensing thin film, MWNTs can effectively catalyze hydrogen peroxide and nanoporous ZrO2 can enhance the stability of the immobilized enzyme. The resulting biosensor provides a very effective matrix for the immobilization of Gox and exhibites a wide linear response range of 8 mmol·L-1 to 3 mmol·L-1 with a correlation coefficient of 0.994 for the detection of glucose. And the response time and detection limit (S/N=3) of the biosensor are determined to be 6 s and 3.5 mmol·L-1, respectively. Another attractive characteristic is that thebiosensor is inexpensive, stable and reliable.

Keywords:nanocomposite, glucose oxidase, nanoporous ZrO2, multiwalled carbon nanotubes, chitosan, biosensors

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