Home > Papers

 
 
Soluble expression and two-step purification of the cytoplasmic domains of Arabidopsis CORYNE in Escherichia coli
Qu Wei 1 * #,Zhao heng 2,Li ShuZhen 3,Luo Yunbo 3
1.School of Biotechnology and Food Engineering, Hefei University of Technology
2.Feed Research Institute, Chinese Academy of Agricultural Sciences
3.College of Food Science and Nutritional Engineering, China Agricultural University
*Correspondence author
#Submitted by
Subject:
Funding: 合肥工业大学青年教师科研启动基金(No.No.GDBJ2009-035)
Opened online: 2 March 2010
Accepted by: none
Citation: Qu Wei,Zhao heng,Li ShuZhen.Soluble expression and two-step purification of the cytoplasmic domains of Arabidopsis CORYNE in Escherichia coli[OL]. [ 2 March 2010] http://en.paper.edu.cn/en_releasepaper/content/40350
 
 
CORYNE (CRN) is a receptor-like protein kinase involved in control of Arabidopsis stem cell proliferation with CLAVATA2. To produce large amounts of soluble cytoplasmic kinase domains of CRN from Escherichia coli, three vectors were constructed using a double tag strategy, named pGEX-CRN, pMAL-CRN and p30-CRN. An auto-induction protocol was used to enhance the overproduction of soluble recombinant CRN with a 32Y media at 28 °C. The production of soluble recombined CRN of these vectors was analyzed and the pGEX-CRN was chosen for large-scale protein production. The dual-tagged GST-CRN-His6 was purified in a two-step affinity chromatography and analyzed for autokinase activity.
Keywords:Arabidopsis;Auto-induction;CRN;Dual-tagged
 
 
 

For this paper

  • PDF (0B)
  • ● Revision 0   
  • ● Print this paper
  • ● Recommend this paper to a friend
  • ● Add to my favorite list

    Saved Papers

    Please enter a name for this paper to be shown in your personalized Saved Papers list

Tags

Add yours

Related Papers

Statistics

PDF Downloaded 271
Bookmarked 0
Recommend 5
Comments Array
Submit your papers