Rapid and Sensitive Visual Detection of Methyl Parathion in Homogeneous Reaction Using Water Soluable Films with Immobilized Cholinesterase

Ma Qianqian; Liu Li; He Jingjing; Miao Yuqing

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Rapid and Sensitive Visual Detection of Methyl Parathion in Homogeneous Reaction Using Water Soluable Films with Immobilized Cholinesterase

Ma Qianqian 1 *,Liu Li 2,He Jingjing 1,Miao Yuqing 1

1.Institute of Physical Chemstry,College of Chemistry and Life Science,Zhejiang Normal University

2.Jinhua Plant Protection Station

*Correspondence author

#Submitted by

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Funding: none

Opened online:27 October 2010

Accepted by: none

Citation: Ma Qianqian,Liu Li,He Jingjing.Rapid and Sensitive Visual Detection of Methyl Parathion in Homogeneous Reaction Using Water Soluable Films with Immobilized Cholinesterase[OL]. [27 October 2010] http://en.paper.edu.cn/en_releasepaper/content/4389046

A rapid and sensitive visual detection of methyl parathion in homogeneous reaction was developed using water soluable poly(vinyl alcohol) (PVA) to immobilize cholinesterase (ChE). When used, a piece of ChE-PVA film is put into the solution and dissolved quickly. The released ChE from enzyme film is mixed and interacted fully with substrates and methyl parathion inhibitors. The color reaction is developed quickly in homogeneous system and the change or diference of color could be observed easily and clearly, while for common colorimetric sticks or strips for pesticides, it is dificult to discern the diference of developed color exactly due to the uneven dying on the surface of supporting matrix. With 10 min of inhibiton and 5 min enzyme reaction, 0.1 ?g/mL concentration of methyl parathion could be observed by visual detection.

Keywords:Acetylcholinesterase; Methyl Parathion; Enzyme assay; Immobilization; PVA

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