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Effect of protein molecular weight on the mass transfer in protein mixing
Asad Ahmed,Chai Chuan,Wu Jiangtao *
State Key Labratory of Multiphase Flow and Power Engineering, Xi\'an Jiaotong University
*Correspondence author
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Funding: none
Opened online: 4 January 2011
Accepted by: none
Citation: Asad Ahmed,Chai Chuan,Wu Jiangtao.Effect of protein molecular weight on the mass transfer in protein mixing[OL]. [ 4 January 2011] http://en.paper.edu.cn/en_releasepaper/content/4401691
 
 
Mixing of protein solution with that of precipitating agent is very important in the protein crystallization experiments. In this work, the interferometry images are recorded during the mixing of two proteins with different molecular weight: lysozyme of ~14.6 kDa and trypsin of ~ 23.3 kDa placed in a Mach-Zehnder interferometer. The protein molecular weight dependence on the competition of the transport process and kinetics at the interface are studied. The concentration profiles of protein solution are calculated to analyze the mass transfer during the mixing process. It is observed that the mass transfer process is more efficient during the mixing of protein with higher molecular weight. In addition, the more rapid concentration changes above the interface suggest that convection may dominate the diffusion. The phenomenon of convection is higher in the protein solutions with higher molecular weight.
Keywords:Mass transfer;Protein mixing; Interface; Diffusion; Mach-Zehnder interferometer
 
 
 

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