p53 siRNA inhibit apoptosis of U2OS cells treated with azurin

YAN Xiaobo; FENG Jie; MIAO Xudong

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p53 siRNA inhibit apoptosis of U2OS cells treated with azurin

YAN Xiaobo 1,FENG Jie 2,MIAO Xudong 1 *

1.the 2nd affiliated hospital,school of medicine,zhejiang university

2.Institute for orthopaedic research,zhejiang university

*Correspondence author

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Funding: This project is supported by the National Nature Science Foundation of China（No.30672138), Nature Science Foundation of Zhejiang province in China）

Opened online:21 February 2011

Accepted by: none

Citation: YAN Xiaobo,FENG Jie,MIAO Xudong.p53 siRNA inhibit apoptosis of U2OS cells treated with azurin[OL]. [21 February 2011] http://en.paper.edu.cn/en_releasepaper/content/4410101

[PURPOSE]: Bacterial redox protein azurin could selectively induce apoptosis of human osteosarcoma U2OS cells. We constructed p53 iRNA to test the function of p53 in the apoptosis-inducing effect of azurin in U2OS cells. [RESULTS]: Cells treated with p53 siRNA+azurin showed more living cells, less apoptosis rate, lower caspase-3 activity and up-regulation of bcl-2, down-regulation of bax compared to cells treated with negative siRNA+azurin. Cells treated with negative siRNA+azurin presented positive TUNEL dying while cells treated with p53 siRNA+azurin presented little positive cells. It suggested that p53 siRNA could inhibit the apopsotis induced by azurin 2 days after the treatment of p53 siRNA+azurin. [CONCLUSION]: Azurin may induce apoptosis through the combination with p53. The decrease of p53 protein did not inhibit cell apoptosis but increase cell apoptosis in U2OS osteosarcoma cells 3 days after the treatment of p53 siRNA. Since U2OS cells are p53 wild type cancer cells. It may be possible that p53 plays the role of oncogene in U2OS osteosarcoma cells. The treatment of azurin may transform the function of p53 from inhibiting apoptosist to inducing apoptosis.

Keywords:p53;siRNA;azurin;transfection

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