A small interfering RNA targeting NF-kappaB p65 alone or combined with 5-FU inhibits the growth of esophageal squmous cell carcinoma in nude mice

TIAN Fang; FAN Tianli; JIANG Yanan; ZHANG Xiaoyan; WANG Xinhua

Chinese︱Feedback︱Save this page

• Elaborating Academic Views 　　　　 • Exchanging Innovative Ideas
• Protecting Intellectual Properties 　　• Fast Sharing Science Papers

Sponsored by the Center for Science and Technology Development of the Ministry of Education
Supervised by Ministry of Education of the People's Republic of China

Home > Papers

A small interfering RNA targeting NF-kappaB p65 alone or combined with 5-FU inhibits the growth of esophageal squmous cell carcinoma in nude mice

TIAN Fang 1 *,FAN Tianli 2,JIANG Yanan 2,ZHANG Xiaoyan 2,WANG Xinhua 3

1.School of Basic Medicine, Zhengzhou University, ZhengZhou 450052

2.School of Basic Medicine, Zhengzhou University

3.The First Affiliated Hospital, Zhengzhou University

*Correspondence author

#Submitted by

Subject:

Funding: Research Fund for the Doctoral Program of Higher Education of China （No.20104101120002）

Opened online:14 November 2011

Accepted by: none

Citation: TIAN Fang,FAN Tianli,JIANG Yanan.A small interfering RNA targeting NF-kappaB p65 alone or combined with 5-FU inhibits the growth of esophageal squmous cell carcinoma in nude mice[OL]. [14 November 2011] http://en.paper.edu.cn/en_releasepaper/content/4448763

NF-κB signaling pathway plays an important role in carcinogenesis. Although constitutive NF-κB activation has been reported in many human tumors, the effect of NF-κB signaling pathway in esophageal squamous cell carcinoma (ESCC) is still poorly understood. To explore the role of NF-κB signaling pathway in ESCC, RNA interference (RNAi) was used to knockdown NF-κB p65 protein level in the ESCC cells and in nude mice. 5-FU was used to investigated whether knockdown NF-κB p65 can potentiate 5-FU's antitumor effect. Animal results indicated that the tumor growth was inhibited in p65 siRNA and p65 siRNA + 5-FU groups compared with control group. Immunohistochemistry, RT-PCR and TUNEL assay showed that p65 siRNA downregulated the expression of p65 and enhanced the sensitivity of EC9706 cells to 5-FU treatment in vivo. Overall, our work indicated that downregulation of p65 can increase the tumor apoptosis and potentiates the effects of 5-FU by suppressing NF-κB signaling pathway. Thus, p65 is an interesting target for ESCC treatment.

Keywords:Esophageal squamous cell carcinoma (ESCC); siRNA; NF-κB signaling pathway; 5-Fluorouraci; nude mice

For this paper

● PDF (0B)
● Revision 0 　　
● Print this paper
● Recommend this paper to a friend
● Add to my favorite list

Saved Papers

Please enter a name for this paper to be shown in your personalized Saved Papers list

Tags

Add yours

Related Papers

Statistics

PDF Downloaded	263
Bookmarked	0
Recommend	5
Comments	Array

Submit your papers

Alert Name:
Alerting to:
Authentication email will be sent to your email address in 24 hours
Frequency:
Email Message Format:	Plain text Graphical(HTML)

Complete the form below and we will recommend the selected titles to your friends on your behalf. * Indicates a required field.
Your name*:
Your email address*:
Recipient's name*:
Recipient's email address*:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find the page(s) useful.

Your name:
Your email address:
Recipient's name:
Recipient's email address:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find this page useful.

Disclaimer: This message was sent to your friend using the "Send it to a friend" facility on the Sciencepaper Online’ WWW site, http://www.paper.edu.cn/en. The Sciencepaper Online is not responsible for the content of this email, and anything said in this email does not necessarily reflect the Sciencepaper Online's views.

	Check out RSS, or use RSS reader to subscribe this item

Saved Papers