Application of peptide retention time in proteome research

SHAO Chen; GAO Youhe

Chinese︱Feedback︱Save this page

• Elaborating Academic Views 　　　　 • Exchanging Innovative Ideas
• Protecting Intellectual Properties 　　• Fast Sharing Science Papers

Sponsored by the Center for Science and Technology Development of the Ministry of Education
Supervised by Ministry of Education of the People's Republic of China

Home > Papers

Application of peptide retention time in proteome research

SHAO Chen * #,GAO Youhe

National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences/School of Basic Medicine, Peking Union Medical College

*Correspondence author

#Submitted by

Subject:

Funding: 高等学校博士学科点专项科研基金资助课题（No.20070023021）, 中国医学科学院基础医学研究所院（所）长基金（No.2009PY05）, 北京市自然科学基金（No.5072037）, 国家自然科学基金（No.30870502）, 国家自然科学基金杰出青年基金（No.30725009）

Opened online: 1 March 2011

Accepted by: none

Citation: SHAO Chen,GAO Youhe.Application of peptide retention time in proteome research[OL]. [ 1 March 2011] http://en.paper.edu.cn/en_releasepaper/content/4411659

Liquid chromatography coupled with mass spectrometry (LC-MS) has been one of the most popular approaches in proteome analysis. As an independent parameter to mass spectrometry information, peptide retention time has been utilized to facilitate protein identification and quantification. In the filed of peptide identification, prediction of the retention time combined with routine tandem mass spectrometry (MS/MS) database searching methods could help improve the confidence of identification. The sensitivity of identification could also be improved by matching peaks with both the accurate mass and retention time in multiple aligned LC-MS runs. Meanwhile, because small changes of liquid chromatography(LC) conditions lead to variability in retention times unavoidably，retention time alignment is crucial to label-free quantification. Additionally，post-translational modifications (PTM) could be identified by combining retention time shifts and mass deviation information.

Keywords:Molecular Biology;Proteomics;Bioinformatics;Chromatography;Retention Time

For this paper

● PDF (0B)
● Revision 0 　　
● Print this paper
● Recommend this paper to a friend
● Add to my favorite list

Saved Papers

Please enter a name for this paper to be shown in your personalized Saved Papers list

Tags

Add yours

Related Papers

Statistics

PDF Downloaded	537
Bookmarked	0
Recommend	5
Comments	Array

Submit your papers

Alert Name:
Alerting to:
Authentication email will be sent to your email address in 24 hours
Frequency:
Email Message Format:	Plain text Graphical(HTML)

Complete the form below and we will recommend the selected titles to your friends on your behalf. * Indicates a required field.
Your name*:
Your email address*:
Recipient's name*:
Recipient's email address*:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find the page(s) useful.

Your name:
Your email address:
Recipient's name:
Recipient's email address:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find this page useful.

Disclaimer: This message was sent to your friend using the "Send it to a friend" facility on the Sciencepaper Online’ WWW site, http://www.paper.edu.cn/en. The Sciencepaper Online is not responsible for the content of this email, and anything said in this email does not necessarily reflect the Sciencepaper Online's views.

	Check out RSS, or use RSS reader to subscribe this item

Saved Papers