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An Amperometric Enzyme Sensor Based on Poly-thionine Modified Platinum Electrode for the Detection of Indole-3-acetic Acid
HUANG Lening,WANG Ruozhong,HUANG Zhigang,XIA Shitou,LI Hesong,XIAO Langtao *
Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultral University
*Correspondence author
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Funding: Natural Science Foundation of China （No.Grant 90817101）, Hunan Provincial Science and Technology Department （No.Grant 09JJ1004）, Research Fund for the Doctoral Program of Higher Education of China （No.grant nos.20070537004）
Opened online:11 March 2011
Accepted by: none
Citation: HUANG Lening,WANG Ruozhong,HUANG Zhigang.An Amperometric Enzyme Sensor Based on Poly-thionine Modified Platinum Electrode for the Detection of Indole-3-acetic Acid[OL]. [11 March 2011] http://en.paper.edu.cn/en_releasepaper/content/4412367
 
 
An electrochemical amperometric enzyme sensor based on poly-thionine modified platinum electrode for the detection of indole-3-acetic acid (IAA) was proposed in this paper. The new sensor was developed mainly according to the oxidation reaction of IAA catalyzed by horseradish peroxidase (HRP). Since this oxidation reaction is substantially the electron transfer process, the current changes when electron transfer reaction occurs on the surface of electrode. Thus, it can provide a general electrochemical method for the direct detection of IAA. The poly-thionine film was produced electrochemically on a platinum electrode in 0.05 M acetate sodium acetate buffer solution (pH 4.5) containing 0.005 M thionine with applied potential of -0.4~1.2 V (versus Ag/AgCl) for about 50 min. After the process of thionine electrodeposit, a blue film of poly thionine was plated on the surface of electrode. HRP was immobilized by means of the glutaraldehyde cross-linking technique. Finally, this HRP-IAA sensor was used to detect IAA. Experimental parameters such as the pH of PBS, HRP incubation temperature and HRP incubation time were optimized. The results indicated that this enzyme sensor had satisfactory linearity with the content of IAA in the range of 1 ng/mL~30 ng/mL with a detection limit of 0.1 ng/mL.
Keywords:Botany; Indole-3-acetic acid (IAA); Horseradish peroxidase (HRP); Enzyme biosensor; platinum electrode
 
 
 

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