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Pre-existing root cementum may promote cementum-like matrix formation in vivo by human periodontal ligament cells
Song Aimei 1 #,Cai Jun 2,Pan Keqing 3,Yang Pishan 3 *
1.The School of Stomatology,Shandong University
2.Shandong Provincial Key Laboratory of Oral Biomedicine,Jinan 250012
3.The School of Stomatology,Shandong University,Jinan 250012
*Correspondence author
#Submitted by
Subject:
Funding: 山东省卫生厅资助课题（No.No.2007HZ048）, 教育部博士点基金新教师基金（No.No.200804221080）
Opened online:20 October 2011
Accepted by: none
Citation: Song Aimei,Cai Jun,Pan Keqing.Pre-existing root cementum may promote cementum-like matrix formation in vivo by human periodontal ligament cells[OL]. [20 October 2011] http://en.paper.edu.cn/en_releasepaper/content/4445717
 
 
Objective: To observe if the healthy in situ root cementum can contribute to the new cementum formation by human periodontal ligament cells (PDLCs). Methods:Root slices were made from healthy premolars.The cementum was preserved in situ in test group; while in control group , the root cementum was scaled completely. Human PDLCs were inoculated on root slices. After 7-day co-culture, 20 root slices of each group were wrapped with polytetrafluoroethylene membrane and transplanted subcutaneously into nude mice. All mice were sacrificed after eight weeks and transplanted specimens were performed for histological observation and immunohistochemistrical stain for osteopontin(OPN) and bone sialoprotein(BSP).Results: 14 of 20 specimens in the test group revealed cementum-like tissue formation on top of the old cementum and no splits were observed between them. In control group, 17 specimens showed fibrous tissue formation along the root surface and different width of splits can be seen between the new fibrous tissue and the dentin surface. Only 3 specimens demonstrated the presence of newly formed thin cementum-like tissue.The newly formed cementum-like tissue were stained positively for both OPN and BSP. Conclusions:The results showed the healthy root cementum can provide stability for the newly formed tissue and enhance the cementum-like tissue formation on top of the old cementum . It suggests that the healthy root cementum may promote the human PDLCs to differentiate towards cementoblasts and healthy root cementum should better be preserved during the periodontal regeneration procedure.
Keywords:Oral medicine; root cementum; human periodontal ligament cells; cementoblasts; differentiation; cementum-like matrix
 
 
 

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