The impact of life stage on the gut microbiota of Holotrichia parallela larvae (Coleoptera: Scarabaeidae)

HUANG Shengwei; ZHANG Hongyu

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The impact of life stage on the gut microbiota of Holotrichia parallela larvae (Coleoptera: Scarabaeidae)

HUANG Shengwei 1 #,ZHANG Hongyu 2 *

1.State Key Laboratory of Agricultural Microbiology, WuHan 430070

2. Institute of Urban and Horticultural Pests, Wuhan, 430070

*Correspondence author

#Submitted by

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Funding: Specialized Research Fund for the Doctoral Program of Higher Education of China （No.No. 200805040010）

Opened online:19 January 2012

Accepted by: none

Citation: HUANG Shengwei,ZHANG Hongyu.The impact of life stage on the gut microbiota of Holotrichia parallela larvae (Coleoptera: Scarabaeidae)[OL]. [19 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4461355

In this paper, we demonstrated the composition and diversity of microbial communities in hindgut of Holotrichia parallela larvae, and investigate how did intestine microbial communities respond to life stages. Methods: Extract total DNA of gut bacteria, construct clone libraries of 16S rDNA, and DGGE was performed to analyze the clone libraries. Sequencing and phylogenetic analysis were performed to determine the difference of intestine microbial communities among different instars. Results: Increases in the species richness and diversity of gut microbiota were observed during larval growth. Bacteroidetes comprised the dominant group in the first instar; however, Firmicutes composed the majority of the hindgut microbiota during the second and third instars. Conclusions: Our results suggestted that the gut's bacterial community changes in response to the host's physiology, possibly to meet the host's ecological needs or physiological demands.

Keywords:Agricultural Entomology and Pest Control; gut microbiology; host physiology; PCR-DGGE; White grub

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