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Isolation, identification and characterization of human intestinal bacteria with the ability to utilize chloramphenicol as the sole source of carbon and energy
Zhao Xin 1 #,Tian Fengwei 2,Wang gang 2,Liu Xiaoming 3,Zhang Qiuxiang 2,Zhang Hao 2,Chen Wei 4 *
1.School of Food Science and Technology, Jiangnan University, JiangSu WuXi 214122
2.School of Food Science and Technology, Jiangnan University, 214122
3.School of Food Science and Technology, Jiangnan University, 214122
4.State Key Laboratory of Food Science and Technology, Jiangnan University, 214122
*Correspondence author
#Submitted by
Subject:
Funding: The National High Technology Research and Development Program of China （No.No. 2011AA100901）
Opened online:23 March 2012
Accepted by: none
Citation: Zhao Xin,Tian Fengwei,Wang gang.Isolation, identification and characterization of human intestinal bacteria with the ability to utilize chloramphenicol as the sole source of carbon and energy[OL]. [23 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4471643
 
 
Five aerobic intestinal bacterial strains that utilized chloramphenicol (CAP) as sole carbon and energy source were isolated from fecal samples collected from healthy volunteers. Based on their morphology, physiological characters, and 16S rDNA sequence analysis, four of the five strains were identified as Klebsiella pneumonia and one as Escherichia fergusonii. The degradation rate of strain I-10-CHL (E. fergusonii) varied with the initial concentration of CAP and the maximum specific substrate removal rate was observed at 25 μg CAP/ml. The pH value also had an effect on the degradation rate of CAP and bacterial growth. A pH of 6.5 was optimal for CAP degradation and growth of strain I-10-CHL (E. fergusonii). In mixed substrate batch cultivations, where CAP was one of the components, glucose, acetamide and CAP were simultaneously utilized. The presence of glucose and acetamide increased the growth and substrate degradation rates of CAP. These results indicate that CAP degrading enzymes are inducible in nature. During incubation with E. fergusonii cells, reduction intermediates (1-p-nitrophenyl -2-amino-1, 3-propanediol) were observed. The products of CAP metabolism by species other than E. fergusonii have not yet been characterized. The strains reducing the antibiotic were chloramphenicol susceptible, indicating that the pathway does not appear to mediate chloramphenicol resistance. The role of this CAP reduction pathway in the physiology of Klebsiella pneumonia and E. fergusonii is unknown. Further understanding of the E. fergusonii CAP reduction pathway will contribute to our knowledge of the diversity of prokaryotic aromatic compounds degradation mechanisms.
Keywords:intestinal bacteria; chloramphenicol; antibiotic; degradation pathway
 
 
 

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