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| Wolbachia are maternally inherited intracellular bacteria that infect a wide range of arthropods and nematodes and are associated with various reproductive abnormalities in their hosts. The infection of Wolbachia in the species of psocid, Liposcelis tricolor, was investigated through Long PCR amplification of wsp gene. The results showed that the population of L. tricolor was positive for Wolbachia. The phylogenetic relationship of Wolbachia presented in L. tricolor and in other insect hosts was also analyzed. The result showed that Wolbachia strain presented in host of L. tricolor belongs to the B-group. Wolbachia infection in host of L. tricolor could be removed through antibiotic treatment. The results of crosses, including ♀w+×♂w+,♀w-×♂w+ ,♀w+×♂w- and ♀w-×♂w-, suggested that the removal of Wolbachia has resulted in lower eggs production of L. Tricolor. The mean embryonic mortalities of offspring produced by L. tricolor without Wolbachia were significantly higher than that of control.
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| Keywords:psocid; endosymbiont; molecular detection; antobiotics treatment |
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