Effect of Tbx1 knock down on cardiac performance in zebrafish

Zhanglifeng; Yuexiang Wang; Jiang qiu; Song Hou-yan; GUI Yonghao

Chinese︱Feedback︱Save this page

• Elaborating Academic Views 　　　　 • Exchanging Innovative Ideas
• Protecting Intellectual Properties 　　• Fast Sharing Science Papers

Sponsored by the Center for Science and Technology Development of the Ministry of Education
Supervised by Ministry of Education of the People's Republic of China

Home > Papers

Effect of Tbx1 knock down on cardiac performance in zebrafish

Zhanglifeng 1 #,Yuexiang Wang 2,Jiang qiu 3,Song Hou-yan 3,GUI Yonghao 4 *

1.Children\'s Hospital of Fudan university, ShangHai 201102

2.key laboratory of molecular medicine, ministry of education,fudan university,shanghai

3.key laboratory of molecular medicine, ministry of education,fudan university,shanghai

4.Children's Hospital of Fudan university

*Correspondence author

#Submitted by

Subject:

Funding: doctoral fund of ministry of education of china（No.No. 200802461110）, National natural science foundation（No.No. 30772352）

Opened online:22 February 2012

Accepted by: none

Citation: Zhanglifeng,Yuexiang Wang,Jiang qiu.Effect of Tbx1 knock down on cardiac performance in zebrafish[OL]. [22 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4464207

In this paper, myocardial performance was explored in Tbx1 morpholino injected zebrafish embryos. In this study, Tbx1 specific morpholino was used to reduce the function of Tbx1 in zebrafish. The differentiation of the myocardial cells was observed using whole mount in situ hybridization. Heart rates were observed and recorded under the microscope from 24 to 72 hours post fertilization (hpf). The cardiac performance was analyzed by measuring ventricular shortening fraction and atrial shortening fraction. Tbx1 morpholino injected embryos were characterized by defects in the pharyngeal arches, otic vesicle, aortic arches and thymus. In addition, Tbx1 knock down reduced the amount of pharyngeal neural crest cells in zebrafish. Abnormal cardiac morphology was visible in nearly 20% of the Tbx1 morpholino injected embryos. The hearts in these embryos did not loop or loop incompletely. Importantly, cardiac performance and heart rate were reduced in Tbx1 morpholino injected embryos. Tbx1 might play an essential role in the development of pharyngeal neural crest cells in zebrafish. Cardiac performance is impaired by Tbx1 knock down in zebrafish.

Keywords:zebrafish; cardiac performance; neural crest cells

For this paper

● PDF (0B)
● Revision 0 　　
● Print this paper
● Recommend this paper to a friend
● Add to my favorite list

Saved Papers

Please enter a name for this paper to be shown in your personalized Saved Papers list

Tags

Add yours

Related Papers

Statistics

PDF Downloaded	288
Bookmarked	0
Recommend	5
Comments	Array

Submit your papers

Alert Name:
Alerting to:
Authentication email will be sent to your email address in 24 hours
Frequency:
Email Message Format:	Plain text Graphical(HTML)

Complete the form below and we will recommend the selected titles to your friends on your behalf. * Indicates a required field.
Your name*:
Your email address*:
Recipient's name*:
Recipient's email address*:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find the page(s) useful.

Your name:
Your email address:
Recipient's name:
Recipient's email address:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find this page useful.

Disclaimer: This message was sent to your friend using the "Send it to a friend" facility on the Sciencepaper Online’ WWW site, http://www.paper.edu.cn/en. The Sciencepaper Online is not responsible for the content of this email, and anything said in this email does not necessarily reflect the Sciencepaper Online's views.

	Check out RSS, or use RSS reader to subscribe this item

Saved Papers