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Synthetic Smac Peptide Enhances Chemo-sensitivity of Bladder Cancer Cells
WANG Jing 1,ZENG Fuqing 2 *,WANG Liang 2 #,ZHU Zhaohui 2,JIANG Guosong 2
1.Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, WuHan 430022
2.Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
*Correspondence author
#Submitted by
Subject:
Funding: Special Scientific Research Foundation for the Doctoral Program of Higher Education from Ministry of Education （No.No. 200804871105）
Opened online:20 February 2012
Accepted by: none
Citation: WANG Jing,ZENG Fuqing,WANG Liang.Synthetic Smac Peptide Enhances Chemo-sensitivity of Bladder Cancer Cells[OL]. [20 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4464743
 
 
The effects of synthetic Smac peptide (SmacN7) on chemotherapy sensitivity of bladder cancer cells were investigated．SmacN7 penetratin peptide was synthesized and delivered into T24 cells． MTT assay was used to evaluate the viability of T24 cells induced by low-dosage of MMC; Flow cytometry was used to analyze the proportions of apoptosis; Western blot was used to detect the expression of XIAP and caspase-3; The activity of caspase-3 was measured and the effect of SmacN7 combined with MMC on T24 cell lines was also determined．The results showed that SmacN7 penetratin peptide could successfully interact with endogenous XIAP, increase the proportions of apoptosis of T24 cell lines induced by low-dosage of MMC in a dose- and time-dependent manner. An obvious down-regulation of XIAP expression and up-regulation of caspase-3 was identified by Western blot. The activity of caspase-3 in experimental group was significantly increased as compared with that in the control group; Combining treated with SmacN7 penetratin peptide，the viability of T24 cells was markedly decreased to 2.22 and 3.61 fold in 24h and 48h respectively． It was concluded that SmacN7 penetratin peptide could act as a cell-permeable IAP inhibitor, inhibit the proliferation, induce apoptosis and enhance the chemo-sensitivity of bladder cancer cells to MMC. These findings indicate that SmacN7 penetratin peptide may be a very promising strategy for bladder cancer treatment when combined with chemotherapy.
Keywords:Smac/DIABLO; synthetic peptide; bladder carcinoma; chemo- sensitivity
 
 
 

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