Inhibitory Effect on Monoamine oxidase of Lactobacillus casei JH23's Metabolite

HE Yiwei; GUO Benheng; CHEN Wei; WU Zhenjun; WU Jiang

Chinese︱Feedback︱Save this page

• Elaborating Academic Views 　　　　 • Exchanging Innovative Ideas
• Protecting Intellectual Properties 　　• Fast Sharing Science Papers

Sponsored by the Center for Science and Technology Development of the Ministry of Education
Supervised by Ministry of Education of the People's Republic of China

Home > Papers

Inhibitory Effect on Monoamine oxidase of Lactobacillus casei JH23's Metabolite

HE Yiwei 1 #,GUO Benheng 2 *,CHEN Wei 3,WU Zhenjun 2,WU Jiang 3

1.State Key Laboratory of Food Science and Technology, Jiangnan University, JiangSu WuXi 214122

2. State Key Laboratory of Dairy Biotechnology,Technology Center of Bright Dairy and Food Co.Ltd.,Shanghai 200436,China

3.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China

*Correspondence author

#Submitted by

Subject:

Funding: 973 Program of Ministry of Science and Technology of PRC（No.No. 2010CB735705）

Opened online:28 March 2012

Accepted by: none

Citation: HE Yiwei,GUO Benheng,CHEN Wei.Inhibitory Effect on Monoamine oxidase of Lactobacillus casei JH23's Metabolite[OL]. [28 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4472390

Among the tested Lactobacillus strains assayed for Inhibitory activity on monoamine oxidase (MAO), the supernatant of Lactobacillus casei JH23 cultured in MRS broth showed strong inhibitory activity. The bioactive components from the supernatant could be fractioned into organic solvent soluble (O) and water soluble ones (W) by extraction with ethyl acetate. Both the organic solvent soluble (O) and water soluble components (W) demonstrated a competitive inhibitory activity to MAO in a dose-dependent manner. The IC50 for the organic solvent soluble components (O) to MAO-A and MAO-B were 1.97mg / ml, 5.67mg / ml, while IC50 for the water soluble components (W) were 1.59mg / ml and 4.83mg / ml respectively. A bioactive fraction w1 was obtained from the water soluble components (W) by sephadex-G15 chromatograph, which the IC50 for MAO-A and MAO-B were 0.327mg/ml and1.13mg/ml .W1 could be further fractioned into 2 peaks with absorbance at 200nm and 300nm respectively by RP-HPLC, and the peak appeared at 200nm had good MAO inhibitory effect. The substance will be prepared by liquid phase and determined structure, which can promote the development of spiritual degeneration disease drug.

Keywords:Lactobacillus casei JH23; Monoamine oxidase (MAO); Inhibition

For this paper

● PDF (0B)
● Revision 0 　　
● Print this paper
● Recommend this paper to a friend
● Add to my favorite list

Saved Papers

Please enter a name for this paper to be shown in your personalized Saved Papers list

Tags

Add yours

Related Papers

Statistics

PDF Downloaded	268
Bookmarked	0
Recommend	5
Comments	Array

Submit your papers

Alert Name:
Alerting to:
Authentication email will be sent to your email address in 24 hours
Frequency:
Email Message Format:	Plain text Graphical(HTML)

Complete the form below and we will recommend the selected titles to your friends on your behalf. * Indicates a required field.
Your name*:
Your email address*:
Recipient's name*:
Recipient's email address*:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find the page(s) useful.

Your name:
Your email address:
Recipient's name:
Recipient's email address:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find this page useful.

Disclaimer: This message was sent to your friend using the "Send it to a friend" facility on the Sciencepaper Online’ WWW site, http://www.paper.edu.cn/en. The Sciencepaper Online is not responsible for the content of this email, and anything said in this email does not necessarily reflect the Sciencepaper Online's views.

	Check out RSS, or use RSS reader to subscribe this item

Saved Papers