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Comparative analysis of nuclear oligomerization domain proteins (NOD)-1 and NOD2 in orange-spotted grouper (Epinephelus coioides)
HOU Qinghua 1,LU Danqi 2 #,YI Shibai 1,DING Xu 1,SUN Yan 1,ZHANG Huixian 1,LIU Xiaochun 1,ZHANG Yong 1,LIN Haoran 1 *
1.State Key Labrotary of Biocontrol, Institute of Aquatic Economic Animals and the Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Science, Sun Yat-Sen University, Guangzhou, China, 510275
2. College of Ocean and Meteorology, Guangdong Ocean University, Zhanjiang, Guangdong, China, 524088.
*Correspondence author
#Submitted by
Subject:
Funding: The 863 Projects (No.No. 2012AA10A407), Guangdong Natural Science Foundation (No.No.10451027501005658), Ph.D. Programs Foundation of Ministry of Education of China), the Fundamental Research Funds for the Central Universities (No.No. 11lgpy64), the Special Support of the China Postdoctoral Science Foundation (No.No. 200902347), the Special Fund for Agro-Scientific Research in Public Interest (No.No.200903046), the National Natural Science Foundation of China (No.No. 30871914 and 31001126)
Opened online:31 December 2012
Accepted by: none
Citation: HOU Qinghua,LU Danqi,YI Shibai.Comparative analysis of nuclear oligomerization domain proteins (NOD)-1 and NOD2 in orange-spotted grouper (Epinephelus coioides)[OL]. [31 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4499074
 
 
Nucleotide-binding oligomerization domain-containing proteins-1 and-2 (NOD1 and NOD2) are members of the NOD-like receptors (NLRs) family. They are both cytoplasmic receptors, and sense microbial infections/danger molecules to induce host innate immune response. In this study, the full-length ORF sequences of NOD1 and NOD2 were cloned, and the putative amino acid sequences were identi?ed in orange-spotted grouper (Epinephelus coioides). The complete open reading frame (ORF) of grouper NOD1 contained 2823 bp encoding a 940 amino acid protein. Grouper NOD2 cDNA contained a 2967 bp ORF, encoding a protein of 988 amino acid residues. Both grouper NOD1 and NOD2 had similar domains to human and fish counterparts. Phylogenetic tree analysis showed that grouper NOD1 clustered with grass carp, zebra?sh and channel cat?sh, while NOD2 was most closely related to fugu. Expression patterns of grouper NOD1 and NOD2 were next studied. NOD1 had the highest level of expression in skin while NOD2 in trunk kidney. Post Vibrio alginolyticus (strain EcGS020401), lipopolysaccharide (LPS) or PolyI:C challenges, gene expression of grouper NOD1 and NOD2 was stimulated to different extents. NOD1 showed a significant enhancement after LPS stimulation, but NOD2 increased more significantly after PolyI:C invasion, indicating that NOD1 and NOD2 may exert different effects on the eradication of bacteria and virus. The adaptor protein RIP-like-interacting CLARP kinase (RICK) and downstream molecule interleukin-8 (IL-8) were also induced at different levels after stimulation, which indicated that NOD1 and NOD2 signal transduction was involved in grouper innate immune protection against bacterial and viral infections.
Keywords:Orange-spotted grouper; NOD1; NOD2; RICK; IL-8
 
 
 

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