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Macrophang inflammatory protein 3 beta promotes survival in rat primary cortical neurons and PC12 cells
MA Jun 1,QIAN Xiying 2,JIN Lide 2,FANG Shaolong 3,FU Guoping 3,CAO Yi 3,XU Wei 3 *,CAO Xia 3 #
1.Department of abdominal tumor surgery, the 3rd affiliated hospital of Kunming Medical University, Kunming 530100
2.Department of neurosurgery, the 1st people's hospital of Yunnan Province, Kunming 650032
3.Department of neurosurgery, the 2nd affiliated hospital, Kunming 650101
*Correspondence author
#Submitted by
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Funding: 教育部博士学科点基金（No.20092317110001）
Opened online:17 December 2012
Accepted by: none
Citation: MA Jun,QIAN Xiying,JIN Lide.Macrophang inflammatory protein 3 beta promotes survival in rat primary cortical neurons and PC12 cells[OL]. [17 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4500497
 
 
Background We have previously reported that CCR7 may be neuroprotective in the central nervous system. This study aimed to investigate whether macrophage inflammatory protein 3 beta (MIP-3 beta /CCL19), which is the ligand for CCR7, could promote survival in primary neurons and PC12 cells in vitro. Results We found that within the concentration range of 50 to 200 ng/ml, MIP-3 beta promoted survival in serum-deprived rat primary cortical neurons as well as reduced serum deprivation-induced Bim expression. However, primary cortical neurons were less viable at higher concentrations of MIP-3 beta (≥300 ng/ml). To investigate whether MIP-3 beta acts through its receptor, CCR7, the pEGFP-N1-CCR7 expression vector was constructed and transfected into PC12 cells. We found that MIP-3 beta enhanced survival of PC12 cells that were transfected with pEGFP-N1-CCR7 in serum-free media. In these transfected PC12 cells, MIP-3 beta increased Akt phosphorylation levels at 15 and 60 min this effect was effectively blocked by wortmannin, a specific PI3K inhibitor. ConclusionsThese data suggest that MIP-3 beta promotes survival in serum-deprived rat primary cortical neurons through its receptor, CCR7, and acts as a neurotrophic factor in PC12 cells, most likely via the PI3K/Akt signaling pathway.
Keywords:chemokine; neuron; apoptosis
 
 
 

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