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The total AvBD-6 gene expressed by Pichia pastoris
Wenyu Sun #,Anshan Shan *,Qingquan Ma,Na Dong,Yanping Cao,Liang Wang,Zeyun Wang,Changjiang Yan
Northeast Agricultural University
*Correspondence author
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Funding: 高等学校博士学科点专项科研基金（No.No.20092325110009）
Opened online:16 January 2013
Accepted by: none
Citation: Wenyu Sun,Anshan Shan,Qingquan Ma.The total AvBD-6 gene expressed by Pichia pastoris[OL]. [16 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4512514
 
 
Avian β-defenisin 6 (AvBD-6) is a 67 amino acid-long peptide that plays an important role in the innate immunity of chickens and is widely expressed in most tissues. In this study, the codon-optimized AvBD-6 gene was synthesized and cloned into the eukaryotic expression vector pPICZαA and expressed in Pichia pastoris. Expression levels of the recombinant AvBD-6 were tested by Tricine-SDS-PAGE, and the product concentration reached 213.0 mg/l after optimization of the expression conditions. Recombinant AvBD-6 exhibited antimicrobial activity against bacteria with the minimal inhibition concentration (MIC) from 5.8 to 46.6 μg/ml and showed no hemolytic activity.
Keywords:antimicrobial peptides; AvBD-6; total gene sequence; expression; Pichia pastoris
 
 
 

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