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Proteomic analysis of human donor liver tissues subjected to ischemia/reperfusion injury during liver transplantation
WU Bin 1 #,WU Hongli 2,CHEN Jianning 3,HUA Xuefeng 1,LI Ning 1,LU Minqiang 1 *
1.Department of liver transplantation, the 3rd affiliated hospital of Sun-Yat-Sen University, Guangzhou 510630
2.Department of veterinary and biomedical sciences, 120 VBS, University of Nebraska-Lincoln, Lincon, USA, 68583-0905
3.Department of pathology, Tianhe road 600, The 3rd affiliated hospital of Sun-Yat- Sen University, Guangzhou, 510630
*Correspondence author
#Submitted by
Subject:
Funding: Guangzhou technology scheming project funding（No.No. 2009Z1-E211）, Doctoral science foundation of Chinese educational ministry（No.No. 20090171110071）
Opened online:27 March 2013
Accepted by: none
Citation: WU Bin,WU Hongli,CHEN Jianning.Proteomic analysis of human donor liver tissues subjected to ischemia/reperfusion injury during liver transplantation[OL]. [27 March 2013] http://en.paper.edu.cn/en_releasepaper/content/4531303
 
 
Purpose: to explore the specific alterations in protein profiles that took place during ischemia/reperfusion injury (I/RI) and find novel therapeutic strategies to reduce I/RI during orthotopic liver transplantation (OLT). Method: we used the comparative proteomic approach of two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to compare the proteomic profiles of the same donor liver at three different time points: T1, immediately after cardiac arrest of donors (normal control). T2, before portal vein was recirculated (ischemia). T3, two hours after the anastomosis of the hepatic artery (reperfusion). Result: we identified 34 proteins that were significantly altered during I/RI. These differentially expressed proteins were functionally classified into seven categories: metabolic enzyme, molecular chaperone, antioxidant enzyme, cytoskeleton protein, signal transduction protein, cyclin, and binding protein. Among the 34 proteins, nine proteins changed only during ischemia (from T1 to T2) period, eleven proteins changed only during reperfusion (from T2 to T3) period, others changed during both ischemia and reperfusion (from T1 to T3) periods. Conclusion: ischemia and reperfusion during LT may lead to different modifications of the liver proteins. Most metabolic enzymes and antioxidant enzymes were up-regulated during ischemia period, indicated that lipidic metabolic disorder and oxidative stress were closely related to the development of ischemia injury. ER chaperones may play a vital role in mediating I/RI and prevention of ER stress caused by I/RI may be used as a key therapeutic target to improve the outcome of LT.
Keywords:liver transportation; ischemia reperfusion injury; protemics
 
 
 

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