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Visual Detection of rpoB Mutations in Rifampin-Resistant Mycobacterium tuberculosis Strains by Use of an Asymmetrically Split Peroxidase DNAzyme
Deng Minggang 1,Feng Shuo 2,Luo Fengling 3,Sun Xiaoming 3,Wang Shaoru 2,Zhou Xiang 2,ZHANG Xiao-Lian 3 * #
1.College of Chemistry and Molecular Sciences, Wuhan University, WuHan 430071
2.College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, People’s Republic of China
3.Department of Immunology, Wuhan University School of Medicine
*Correspondence author
#Submitted by
Subject:
Funding: the 973 Program of China (No.No. 2009CB522507), the National Special Fund of China for Important Infectious Disease (No.No. 2012ZX-10003-002), the National Natural Science Foundation of China (No.No. 30921001 and 30800038), the Program for Changjiang Scholars and Innovative Research Team at University, the 211 program (No.No. 303-581045), The National Outstanding Youth Foundation of China (No.No. 81025008)
Opened online:10 April 2013
Accepted by: none
Citation: Deng Minggang,Feng Shuo,Luo Fengling.Visual Detection of rpoB Mutations in Rifampin-Resistant Mycobacterium tuberculosis Strains by Use of an Asymmetrically Split Peroxidase DNAzyme[OL]. [10 April 2013] http://en.paper.edu.cn/en_releasepaper/content/4531343
 
 
Multidrug-resistant Mycobacterium tuberculosisis resistant to two first-line antituberculosis drugs, isoniazid and rifampin, resulting in the relapse of tuberculosis. M. tuberculosisgrows very slowly, and thus traditional examination methods take time to test its drug resistance and cannot meet clinical needs. The use of a DNA probe makes it possible to test rifampin resistance. Wedeveloped an asymmetrical split-assembly DNA peroxidase assay to detect drug-resistant mutation of rifampin-resistant M. tuberculosisin the rpoBgene rapidly and visibly. A new strategy was also designed to eliminate the adverse effects caused by the complicated secondary structure of the target DNA and to improve the efficiency of the probes. This detection system consists of five group detections, covers rifampin-resistant determination region of therpoBgene, and tests 40 kinds of mutations, including the most common mutations at codons 531 and 526. Every group detection or individual mutant allele detection can distinguish corresponding mutant DNA sequences from the wild-type DNA sequences.
Keywords:Mycobacterium tuberculosis; DNA probes; Peroxidase DNAzyme
 
 
 

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