The Protective Effect of ATP on Skeletal Muscle Satellite Cells Damaged by H2O2

FEI Fei; ZHU Daoli; TAO Lijun; HUANG Baozhu; ZHANG Honghong

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The Protective Effect of ATP on Skeletal Muscle Satellite Cells Damaged by H2O2

FEI Fei 1,ZHU Daoli 2 * #,TAO Lijun 3,HUANG Baozhu 4,ZHANG Honghong 5

1.School of Life Science, Nantong University, JiangSu Nantong 226019

2.School of Life Science,Natong University, Nantong City 226019

3.School of Life Science, Nantong University, Natong City 226019

4.School of Life Science, Natong University, Nantong City 226019

5.School of Life Science, Nantong University, Nantong City 226019

*Correspondence author

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Funding: This Work was supported by grants from the building special project of Nantong University doctor‘s spot（No.05024276）, Grant: The technology plan was supported by the social development science of Nantong City science and technology burrau（No.S2008022）

Opened online:27 April 2013

Accepted by: none

Citation: FEI Fei,ZHU Daoli,TAO Lijun.The Protective Effect of ATP on Skeletal Muscle Satellite Cells Damaged by H2O2[OL]. [27 April 2013] http://en.paper.edu.cn/en_releasepaper/content/4539356

Objective To investigate the protective effect of ATP on skeletal muscle satellite cells of newborn rats damaged by H2O2 and its influence on the related proteins Bax and Bcl-2. Methods The H2O2-induced damage models of skeletal muscle satellite cells of newborn rats were established and the study was divided into 4 groups：normal control group，model control group (0.1 mmol / L H2O2 solution damaged cells for 50s)，protection group(10，5，2.5，1.25，0.625，0.3125，0.15625 mg/mL ATP solutions treated cells and then 0.1 mmol / L H2O2 solution damaged cells for 50s)，proliferation group (10，5，2.5，1.25，0.625，0.3125，0.15625 mg/mL ATP solutions treated cells). MTT colorimetric analysis，FITC+PI+DAPI fluorescent staining，Giemsa staining and immunocytochemistry fluorescent apoptotic antibody test were applied to detect the survival rate of cells and the protective effect of ATP on skeletal muscle satellite cells damaged by H2O2. Results The survival rate of skeletal muscle satellite cells was decreased and the apoptosis rate was increased after damaged. The survival rate of muscle satellite cells protected by the ATP solution could be increased (P<0.01), and the apoptosis rate was decreased. Immunocytochemistry staining analysis results showed that ATP solution could enhance Bcl-2 proteins (P<0.01) and inhibite Bax proteins (P<0.01). Different doses of ATP solutions had different effects on skeletal muscle satellite cells induced by H2O2: the survival rate of muscle satellite cells protected by the adequate doses of ATP solutions （2.5、1.25、0.625 mg/mL）could be increased. The protective effect on cells treated by 1.25 mg/mL ATP solution was the most obvious. Cells treated by 10，5mg/mL ATP solutions could even cause obvious apoptosis. Conclusion The ATP solution had obvious protective effect on skeletal muscle satellite cells damaged by H2O2 in new-born rats. The main mechanism of ATP solution was related to strengthen the expression of Bcl-2 and inhibit that of Bax through JAK -STAT signal transduction, thus inhibiting apoptosis of cells.

Keywords:Adenosine-triphosphate; Hydrogen peroxide; Damage and protection; Skeletal muscle satellite cells; Bcl-2; Bax

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