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Effects of Magnetic Nanoparticle of Fe3O4 and 5Bromotetrandrin on apoptosis induced by Daunorubicin in K562/A02 Leukemic cells
Shen Mingfang 1 #,Chen Baoan 2 *,Cheng Jian 2,Gao Feng 2,Xu Wenlin 3,Ding Jiahua 2,Gao Chong 2,Sun Xinchen 4,Li Guohong 2,Chen Wenji 2,Liu Lijie 5,Li Xiaomao 6,Wang Xuemei 7
1.Department of Hematology, Zhongda Hospital
2. Department of Hematology, Zhongda Hospital
3.Department of Hematology, People’s Hospital
4.Department of Oncology, Zhongda Hospital
5.Institution of Physiology, Southeast University
6.Department of Physics, University of Saarland
7.National Key Lab of Bioelectronics(Chien-Shiung Wu Laboratory), Southeast University
*Correspondence author
#Submitted by
Subject:
Funding: 国家自然基金,国家自然基金,高等学校博士学科点专项科研基金,国家863计划(No.30740062,30872970,20070286059,2007AA0222006)
Opened online:23 January 2009
Accepted by: none
Citation: Shen Mingfang,Chen Baoan,Cheng Jian.Effects of Magnetic Nanoparticle of Fe3O4 and 5Bromotetrandrin on apoptosis induced by Daunorubicin in K562/A02 Leukemic cells[OL]. [23 January 2009] http://en.paper.edu.cn/en_releasepaper/content/28301
 
 
The aim of this study was to investigate the potential benefit of combination therapy with Magnetic Nanoparticle of Fe3O4 and 5Bromotetrandrine on chronic leukemia. We detected the apoptosis by flow cytometry(FCM) and Wright staining. The expression of BAX and BCL-2 was measured by Western blot.The results showed that combination of DNR with either MNP(Fe3O4) or 5-BrTet exerted a potent cytotoxic effect on K562/A02 cells, while MNP(Fe3O4) and 5-BrTet cotreatment could synergistically enhance DNR-induced apoptosis. After treated with this composite, K562/A02 cells appeared typical apoptotic morphological features; the expression level of BCL-2 decreased and BAX increased markedly.It is concluded that MNP(Fe3O4) or 5-BrTet with DNR induced apoptosis in K562/ A02 cells, and when used together,they had distinct synergism .The down-regulation of BCL-2 and the up-regulation of BAX may play important roles.
Keywords:K562/A02 leukemic cells;multidrug resistance;Magnetic Nanoparticle of Fe3O4;5Bromotetrandrine;apoptosis;BCL-2;BAX
 
 
 

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