Molecular Cloning and Characterization of the First Caspase in the Striped Stem Borer, Chilo suppressalis

LU Mingxing; DUYuzhou; CAO Shuangshuang; LIU Pingyang; LI Jianyong

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Molecular Cloning and Characterization of the First Caspase in the Striped Stem Borer, Chilo suppressalis

LU Mingxing 1 #,DUYuzhou 2,CAO Shuangshuang 3,LIU Pingyang 4,LI Jianyong 5 *

1.College of Horticulture and Plant Protection & Institute of Appled Entomology, Yangzhou University, Yangzhou 225009

2. Department of Biochemistry, Virginia Tech, Blacksburg 24061 VA, USA

3.College of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou 225000

4.College of Horticulture and Plant Protection & Institute of Appled Entomoogy, Yangzhou University, Yangzhou 225000

5.Department of Biochemistry, Virginia Tech, Blacksburg 24061VA , USA

*Correspondence author

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Funding: This work was supported by the National Basic Research and Development Program of China （No.2012CB114100 ）, Specialized Research Found for the Doctoral Program of Higher Educatio（No.20113250110008）

Opened online:23 May 2013

Accepted by: none

Citation: LU Mingxing,DUYuzhou,CAO Shuangshuang.Molecular Cloning and Characterization of the First Caspase in the Striped Stem Borer, Chilo suppressalis[OL]. [23 May 2013] http://en.paper.edu.cn/en_releasepaper/content/4543623

Apoptosis is executed through the activity of the caspases that are aspartyl-specific proteases. In this study, we isolated the caspase gene (Cscaspase-1) of Chilo suppressalis (one of the leading pests responsible for destruction of rice crops). It possesses the open reading frame (ORF) of 295 amino acids including prodomain, large subunit and small subunits, and two cleavage sites (Asp23 and Asp194) were found to be located among them. In addition to these profiles, Cscaspase-1 contains two active sites (His134 and Cys176). Genomic analysis demonstrated there was no intron in the genome of Cscaspase-1. The Cscaspase-1 transcripts were found in all tissues of the fifth instar larvae, and higher levels were found in the midgut, hindgut and Malpighian tubules. Examination of Cscaspase-1 expression in different developmental stages indicated low constitutive levels in the eggs and early larvae stages, and higher abundances were exhibited in the last larvae and pupae stages. The relative mRNA levels of Cscaspase-1 were induced by heat and cold temperatures. For example, the highest increase of Cscaspase-1 transcription was at ?3 C and 36 C respectively. In a word, Cscaspase-1 plays a role of effector in the apoptosis of C. suppressalis. It also correlates with development, metamorphosis and thermotolerance of C. suppreassalis.

Keywords:Cscaspase-1; Chilo suppressalis; expression

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