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Biological behavior of neurally differentiated periodontal ligament stem cells on different titanium implant surfaces
LI Xiaojie 1,LIAO Dapeng 2 * #,GONG Ping 3,DONG Yan 2,SUN Gang 2
1.Department of Dentistry, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016
2.Department of Dentistry,2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hang zhou, 310009
3.Dental Implant Center,West China College of Stomatology,Sichuan University
*Correspondence author
#Submitted by
Subject:
Funding: none
Opened online:14 June 2013
Accepted by: none
Citation: LI Xiaojie,LIAO Dapeng,GONG Ping.Biological behavior of neurally differentiated periodontal ligament stem cells on different titanium implant surfaces[OL]. [14 June 2013] http://en.paper.edu.cn/en_releasepaper/content/4547249
 
 
Objective: We investigated the biological behavior of periodontal ligament stem cells (PDLSCs) induced to differentiate into Schwann cells (SCs) on the surfaces of titanium discs. Methods: Two types of titanium surfaces, sandblasted and acid-etched (SLA) and smooth polished, were prepared on titanium discs, and differentiated PDLSCs were seeded onto them. Cell morphology was examined by scanning electron microscopy, and cell proliferation was assessed using assays for MTT metabolism and total protein content. RT-PCR and Western blotting analyses were used to determine the gene and protein expression, respectively, of SC markers by PDLSCs. Results: Differentiated PDLSCs could adhere, proliferate, differentiate, and express SC marker genes and proteins on the prepared titanium surfaces, and the highest levels of SC marker protein expression were observed in PDLSCs on SLA titanium surfaces. Conclusions: SLA titanium surfaces show good biocompatibility for differentiated PDLSCs, which is important for the application of these cells in peri-implant nerve tissue engineering, through which they are expected to improve the osseoperception of dental implants.
Keywords:Periodontal ligament stem cells; Schwann cells; differentiation; titanium surface; biological behavior
 
 
 

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