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Apigenin, being an important dietary compound, is a natural product mainly contained in many vegetables. Growing evidence reveals that the intake of apigenin, which is rich source of celery, is negatively associated with various diseases. However, high-efficient extraction of apigenin, with low cost from complex-component plant vegetable tissue, is still a problem. In this study, the influence of different operational conditions during ultrasonic-assisted beta-glucosidase hydrolysis on apigenin extraction from celery was addressed. Enzymatic hydrolysis showed high apigenin extraction capacity at 0.1 mg/mL concentration, after 15 h, at pH 6. Ultrasonic-assisted enzymatic hydrolysis also achieved high extraction activity towards apigenin with these optimal parameters: an exposure period of 40 min at 60C, power source 60 W, and with 70% methanol concentration |
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Keywords:Apigenin extraction; beta-Glucosidase hydrolysis; Ultrasonic-assisted; Operational conditions |
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