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A simple flourescence spectroscopic approach for simultaneous and rapid detection of four polycyclic aromatic hydrocarbons (PAH4) in vegetable oil
Liu Yi-Hong 1,Wu Ping-Ping 1,Liu Qian 1,Luo He-Dong 2,Cao Shuo-Hui 3,Lin Guo-Chun 4,Fu Dong-Sai 1,Zhong Xiu-Di 1,Li Yao-Qun 1 * #
1.Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China
2.Xiamen Municipal Center for Disease Control and Prevention, Xiamen, 361021, China
3.Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China; Department of Electronic Science Shcool of Physics and Mechanical & Electrical Engineering, Xiamen University, Xiamen, 361005, China
4.Department of Electronic Science Shcool of Physics and Mechanical & Electrical Engineering, Xiamen University, Xiamen, 361005, China
*Correspondence author
#Submitted by
Subject:
Funding: National Natural Science Foudantion of China(No.21375111, 21127005, and 11474239), The Ministry of Education of China (No.20110121110011 and IRT13036)
Opened online: 1 December 2015
Accepted by: none
Citation: Liu Yi-Hong,Wu Ping-Ping,Liu Qian.A simple flourescence spectroscopic approach for simultaneous and rapid detection of four polycyclic aromatic hydrocarbons (PAH4) in vegetable oil[OL]. [ 1 December 2015] http://en.paper.edu.cn/en_releasepaper/content/4662252
 
 
Based on the conclusions of United States Environmental Protection Agency (USEPA), the four polynulear aromatic hydrocarbons (PAH4) Chrysene, benzo(a)pyrene (BaP), benzo(a)anthracene (BaA), and benzo(b)fluoranthene (BbF) are new markers for the occurrence of PAHs in food. Vegetable oil is a type of food which easy to be contaminated by PAHs due to the raw material pollution and the imperfections in processing technology. Hence, it is of importance to establish efficient method for rapid determination of PAH4 in vegetable oil. In this research, second-derivative nonlinear variable-angle-matrix isopotential synchronous fluorescence spectroscopic approach has been proposed for simultaneous and rapid determination of Chrysene, BaA, BaP and BbF in vegetable oil with ultrasonic-assisted extraction. This method is capable of resolving the spectra of four PAHs well. The limits of detection (LODs) for Chrysene, BaA, BaP and BbF were 13.0, 8.64, 0.16 and 7.68 μg/kg, respectively. The recoveries in various oil samples for Chrysene, BaA, BaP and BbF were 86.2 ± 15.3, 93.8 ± 11.7, 93.9 ± 12.1 and 94.3 ± 7.4 %, respectively, at the spiked levels of 10 ~ 400 μg/kg. The relative standard deviations (RSDs) achieved for Chrysene, BaA, BaP and BbF were 3.0, 8.4, 6.3 and 7.2 %, respectively. The established method is simple, economical and reliable, and provides an attractive alternative for the simultaneous and rapid determination PAH4 in vegetable oil samples.
Keywords:Synchronous fluorescence spectroscopy; ultrasonic-assisted extraction; vegetable oil; Chrysene; benzo(a)anthracene; benzo(a)pyrene; benzo(b)fluoranthene
 
 
 

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