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Comparative transcript profiling of gene expression between self-incompatibility and self-compatibility mandarin by suppression subtractive hybridization and cDNA microarray
Miao Hongxia 1,Ye Zixing 2,Hu Guibing 1,Qin Yonghua 3 * #
1.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Horticulture, South China Agricultural University, Guangzhou 510642, China
2.Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou, 510642, China
3. Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou, 510642, China
*Correspondence author
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Funding: This work was supported by National Natural Science Foundation of China （No.No. 31000899), Research Fund for the Doctoral Program of Higher Education of China）
Opened online:30 December 2013
Accepted by: none
Citation: Miao Hongxia,Ye Zixing,Hu Guibing.Comparative transcript profiling of gene expression between self-incompatibility and self-compatibility mandarin by suppression subtractive hybridization and cDNA microarray[OL]. [30 December 2013] http://en.paper.edu.cn/en_releasepaper/content/4575123
 
 
Self-incompatibility (SI) is an important trait of Citrus plants that is exploited by farmers for producing seedless fruit in Citrus. However, the molecular mechanism of SI in Citrus is not well understood. 'Wuzishatangju' (Citrus reticulata Blanco) (Self-incompatibility, SI) is an excellent seedless mandarin cultivar derived from a bud sport variation of a seedy 'Shatangju' cultivar (Self-compatibility, SC). Therefore, the two cultivars are excellent materials to study the mechanisms of SI and/or SC in Citrus. An integrative strategy combining eight suppression subtractive hybridization (SSH) libraries with cDNA microarray was used to study the different molecular mechanisms between SI 'Wuzishatangju' and SC 'Shatangju' (control) mandarins. A custom microarray screen resulted in a total of 1820 up- or down-regulated (Ratio≥3.0) obtained from 9810 positive clones. The expression of genes involved in embryonic development, ubiquitination pathway, Ca2+-signaling pathway, gibberellins (GAs) and auxin was significantly up-regulated in SI 'Wuzishatangju' compared with SC 'Shatangju' mandarin. The microarray analysis suggests that ubiquitin mediated proteolysis pathway might be involved in the SI reaction of 'Wuzishatangju.' Our research highlighted some candidate pathways that participate in the mandarin SI/SC reaction and could be beneficial for understanding the SI/SC systems evolution and breeding seedless citrus fruits in the future.
Keywords:Citrus reticulata Blanco; Self-incompatibility; SSH; cDNA microarray; Expression analysis
 
 
 

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