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| Insect prophenoloxidase (PPO) is a copper-containing enzyme, playing an important role in immunity reaction. In this paper, the expression of PxPPO1 at different life stages were investigated, Semi-quantitative PCR and Real-time quantitative PCR indicated that the highest amount of PxPPO1 transcripts in eggs and the 4th instar larvae, followed by the 2nd, the 3rd instar larvae, prepupae and pupa. The ORF of PxPPO1 was cloned and inserted into pET-30a prokaryotic expression vector, the recombinant proteins was expressed in Escherichia coli, and was confirmed with an 83 kDa visible, but insoluble protein. |
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| Keywords:Plutella xylostella; Prophenoloxidase; PxPPO1 expression; developmental stages |
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