Heat shock treatment regulates the expression of NFKBIA (IκBα) in a post-transcriptional manner

Mei Zhuzhong; Li Tao; Wang Ni; Chen Xinyu; Ou Xiaoli; Jiang Yong

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Heat shock treatment regulates the expression of NFKBIA (IκBα) in a post-transcriptional manner

Mei Zhuzhong * #,Li Tao,Wang Ni,Chen Xinyu,Ou Xiaoli,Jiang Yong

Key Laboratory of Functional Proteomics of Guangdong Province, Department of Pathophysiology, Southern Medical University, Southern Medical University，Guangzhou 510515，China

*Correspondence author

#Submitted by

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Funding: 国家自然科学基金（No.81070955）, 教育部新教师基金项目（No.20104433120009）

Opened online:15 January 2014

Accepted by: none

Citation: Mei Zhuzhong,Li Tao,Wang Ni.Heat shock treatment regulates the expression of NFKBIA (IκBα) in a post-transcriptional manner[OL]. [15 January 2014] http://en.paper.edu.cn/en_releasepaper/content/4581250

IκBα, encoded by NFKBIA, is the prototype inhibitor of transcription factor NF-κB. Notably, the transcription of NFKBIA is directly regulated by NF-κB. In contrast, the contribution of post-transcriptional regulation in the expression of NFKBIA remains obscure. To elucidate the post-transcriptional regulation of NFKBIA, we first aligned the 3?-UTR from several different species (human, mouse, rat, dog and cow). The alignment results revealed that the 3?-UTR sequences were highly conserved among these analyzed species. Dual luciferase assay analysis showed that 3?-UTR of mouse NFKBIA downregulated the expression of associated luciferase gene. Heat shock treatment stabilized NFKBIA mRNA and promote the expression of luciferase gene associated with mouse NFKBIA 3?-UTR. Biotin-labeled RNA pulldown assay followed mass spectrometry analysis identified specific binding proteins to the 3?-UTR of mouse NFKBIA mRNA. Half of them were identified as ribosomal proteins of 40S and 60S ribosome subunits.

Keywords:NFKBIA；post-transcriptional regulation；RNA binding protein

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