Optimization of Fermentation Medium of K.rhizophila.H5401 for Producing (R)-Adrenaline Using Response Surface Methodology

CHEN Bingmei; XU Xiaoping

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Optimization of Fermentation Medium of K.rhizophila.H5401 for Producing (R)-Adrenaline Using Response Surface Methodology

CHEN Bingmei,XU Xiaoping *

College of Chemistry and Chemical Engineeing, Fuzhou University, Fujian 350116，China

*Correspondence author

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Funding: Specialized Research Fund for the Doctoral Program of Higher Education（No.No.20103514110002）

Opened online:25 February 2014

Accepted by: none

Citation: CHEN Bingmei,XU Xiaoping.Optimization of Fermentation Medium of K.rhizophila.H5401 for Producing (R)-Adrenaline Using Response Surface Methodology[OL]. [25 February 2014] http://en.paper.edu.cn/en_releasepaper/content/4583994

A bacterium strain Kocuria rhizophila.XL11 capable of producing (R)-adrenaline ((R)-epinephrine) with high optical purity (enantiomeric excess > 99%) was firstly isolated. Optimization of the fermentation medium was performed by using response surface methodology. Critical nutrients were initially selected according to a Plackett-Burman design, followed by an application of steepest ascent method to approach the optimal response area. The optimized medium composition for maximum adrenaline production by the mutant strain Kocuria rhizophila.H5401 was obtained based on a three-level three-factor Box-Behnken design, and the optimum concentrations of the critical factors were obtained as follows: glycerol 8.61 goL-1, peptone 7.22 goL-1 and NH4H2PO4 5.65 goL-1. The maximum yield of adrenaline was found to be 61.14%, which is an increase of 25.0% compared to that obtained from the original fermentation medium.

Keywords:(R)-adrenaline; biotransformation; response surface methodology

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