| |
| In this paper, water quality and bacterial community diversity was determined in a seasonal layered JIN PEN drinking water reservoir (Northwest China) using Illumina Miseq high-throughout sequencing targeting the V6 region of the 16S rRNA gene. Water samples were collected from 0.5, 5, 10, 20, 25, 30, 45, 50, 55 and 65 m depth of JIN PEN reservoir during thermal stratification. The results showed that epilimnion, metalimnion and hypolimnion were formed steadily in JIN PEN reservoir. The temperature was decreased steadily from the surface with 23.11℃ to the bottom with 9.17℃. Total nitrogen (TN) from 1.07 to 2.06 mg/L and nitrate nitrogen ranged from 0.8 to 1.84 mg/L. The dissolved oxygen (DO) concentration was decreased sharply below 50 m, and reached to zero in 65 m. The Miseq sequencing revealed a total of 4127 operational taxonomic units (OTUs) with 97% similarity, which were affiliated with 15 phyla including Acidobacteria, Actinobacteria, Armatimonadetes, Bacteroidetes, Caldiserica, Chlamydiae, Chlorobi, Chloroflexi, Cyanobacteria, Firmicutes, Gemmatimonadetes, Nitrospirae, Planctomycetes, Proteobacteria, and Verrucomicrobia. The highest Shannon diversity was 4.41 in 45 m, and the highest Chao 1 diversity was 506 in 5 m. Rhodobacter was dominated in 55 m (23.24%) and 65 m (12.58%). Prosthecobacter was dominated in 0.5-50 m. The heat map fingerprint and redundancy analysis (RDA) indicated significant difference in vertical water bacterial community composition in the reservoir. Meanwhile, water quality properties including dissolved oxygen, conductivity, nitrate nitrogen and total nitrogen have dramatically influence on the water bacterial community compositions. The data set yields new insights into the relationship between water quality and bacterial community compositions during reservoir thermal stratified periods. |
|
| Keywords:reservoir; thermal stratification; water bacterial community; high-throughput sequencing |
|
