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Effects of p-chlorophenoxyisobutyric acid, arabinogalactan, and activated charcoal on microspore embryogenesis in kale
Niu Ruiqing,Zhang Yun,Ren Jie,Tong Yan,Liu Zhiyong,Wang Yushu,Feng Hui *
College of Horticulture, Shenyang Agricultural University, 110866
*Correspondence author
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Funding: Research Fund for the Doctoral Program of Higher Education of China （No.#20122103110006）, China Agriculture Research System（No.#CARS-25-A-03）
Opened online:10 June 2015
Accepted by: none
Citation: Niu Ruiqing,Zhang Yun,Ren Jie.Effects of p-chlorophenoxyisobutyric acid, arabinogalactan, and activated charcoal on microspore embryogenesis in kale[OL]. [10 June 2015] http://en.paper.edu.cn/en_releasepaper/content/4646235
 
 
To improve embryogenesis in microspore cultures of kale (Brassica oleracea L. var. acephala DC.), 6-benzylaminopurine (6-BA), naphthaleneacetic acid (NAA), arabinogalactan (AG), p-chlorophenoxyisobutyric acid (PCIB), and activated charcoal (AC) were added to the medium using four varieties of kale. The results showed that the addition of AG (0.1-0.2 g/L), AC (0.1-0.2 g/L) or a combination of 6-BA (0.1-0.2 mg/L) and NAA (0.1-0.2 mg/L) promoted embryogenesis. Adding 40 μM PCIB or a combination of 40 μM PCIB and 0.2 g/L AC to NLN-13 medium at pH 5.8 effectively enhanced embryogenesis. Treatment with a combination of 40 μM PCIB and 10 mg/L AG gave the highest rate of embryonic induction, especially in genotype "Y007," which showed a twelve-fold increase in yield.
Keywords: Kale; Embryogenesis; p-Chlorophenoxyisobutyric acid; Arabinogalactan; Activated charcoal
 
 
 

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