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Isolation, Purification and Identification of Torularhodin from Sporidiobolus pararoseus by Column Chromatography and its Anti-Cancer Effect on LNCaP Cells
DU Chao #,SHI Qiuyu,WU Jialing,CUI Yan,LI Yingchao,WEI Liangxin,QIAN He *
School of Food Science and Technology, Jiangnan University, 214122
*Correspondence author
#Submitted by
Subject:
Funding: This article is supported by National Key Technology R&D Program in the 12th Five year Plan of China (No.No.2014BAD04B03, No.2012BAD36B02 and No.2015BAD16B01) and 2015 Independent Research Program of Jiangnan University - Key Projects)
Opened online:27 October 2015
Accepted by: none
Citation: DU Chao,SHI Qiuyu,WU Jialing.Isolation, Purification and Identification of Torularhodin from Sporidiobolus pararoseus by Column Chromatography and its Anti-Cancer Effect on LNCaP Cells[OL]. [27 October 2015] http://en.paper.edu.cn/en_releasepaper/content/4658372
 
 
In the present study, an isolation and purification method of torularhodin from the fermentation products of Sporidiobolus pararoseus by column chromatography was developed. The purity of the pigment was determined to be above 96% by High Performance Liquid Chromatography (HPLC). The spectroscopic data of torularhodin were obtained by HPLC-Diode Array Detector (DAD), Liquid Chromatography (LC) - Electronic Spray Ion (ESI) - Mass Spectrometry (MS), Fourier - Transform Infrared spectroscopy (FTIR), and Raman spectroscopy. This study was also designed to elucidate the inhibition of torularhodin on the growth of prostate cancer LNCaP cells. Torularhodin significantly suppressed the proliferation of LNCaP cells in a dose-dependent manner. After the treatment with 30 μmol β-carotene, lycopene and torularhodin for 24 h, the growth was decreased by 20.5 ± 3.1%, 27.5 ±3.7%, and 60.2 ±1.8% as showed in WST-1 assay. Therefore, torularhodin deserves further study as a potential method to prevent certain kinds of cancer.
Keywords:Food science and technology basic subjects; Sporidiobolus pararoseus; Torularhodin; Column chromatography; Spectroscopic data; Prostate cancer
 
 
 

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