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One step preparation of magnetic dual-template molecularly imprinted nanoparticles for specific recognition of two proteins
ZHAO Siqi 1 #,TANG Yuhai 1,HAO Yi 2,GAO Ruixia 1 *,ZHANG Min 3
1.Department of Chemisrty, School of Science, Xi\'an Jiaotong University, Xi’an, 710049
2.College of Pharmacy, Xi'an Jiaotong University, Xi'an 710049
3.College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai, 200336
*Correspondence author
#Submitted by
Subject:
Funding: the National Natural Science Foundation of China （No.Nos. 21305107，21305086）
Opened online: 5 January 2016
Accepted by: none
Citation: ZHAO Siqi,TANG Yuhai,HAO Yi.One step preparation of magnetic dual-template molecularly imprinted nanoparticles for specific recognition of two proteins[OL]. [ 5 January 2016] http://en.paper.edu.cn/en_releasepaper/content/4674085
 
 
In this paper, a simple one-step imprinted strategy to prepare the core-shell protein imprinted magnetic nanoparticles (DMIPs), based on bovine hemoglobin (BHb) and bovine serum albumin (BSA) as dual templates as well as dopamine (DA) as the monomer and cross-linker, for efficient identification of two high-abundant proteins was described. BHb and BSA were imprinted on the surface of magnetic nanoparticles successively using self-polymerization of DA and surface imprinting technique. The resulting magnetic imprinted nanomaterials were characterized by transmission electron microscope (TEM), X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, and vibrating sample magnetometer (VSM). The results showed that the uniform MIP layer of 8 nm was homogeneously grafted on the surface of magnetic nanoparticles. Moreover, the DMIPs exhibited super-paramagnetic property, resulting in the convenient and highly efficient extraction. A series of adsorption experiments were performed to study the properties of the synthesized DMIPs. The results showed that the DMIPs exhibited the obvious great adsorption performance and satisfactory selectivity for BSA and BHb, which confirmed the effectiveness and feasibility of the dual-template imprinted strategy we proposed. In addition, the as-synthesized nanoparticles could be used for six cycles of adsorption-elution without obvious deterioration, indicating a potential value for simultaneously removal of high-abundant proteins.
Keywords:Analytical chemistry; Dual templates; Proteins; Dopamine; Surface imprinting technique
 
 
 

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