Engineering Sinorhizobium meliloti hemoprotein to enhance nosiheptide production in Streptomyces actuosus

Qiuyan Mo; Huanyuan Zhang; Xin Tang; Lina Zhao; Yuanda Song

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Engineering Sinorhizobium meliloti hemoprotein to enhance nosiheptide production in Streptomyces actuosus

Qiuyan Mo 1,Huanyuan Zhang 2,Xin Tang 1,Lina Zhao 1,Yuanda Song 1 *

1.State Key Laboratory of Food Science and Health, School of Food Science and Technology, Jiangnan University, Wuxi, P.R. China

2.Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, P.R. China

*Correspondence author

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Funding: none

Opened online: 6 April 2016

Accepted by: none

Citation: Qiuyan Mo,Huanyuan Zhang,Xin Tang.Engineering Sinorhizobium meliloti hemoprotein to enhance nosiheptide production in Streptomyces actuosus[OL]. [ 6 April 2016] http://en.paper.edu.cn/en_releasepaper/content/4681619

Oxygen deficiency is a critical limiting factor for nosiheptide production in Streptomyces actuosus during fermentation. To alleviate oxygen limitation and enhance the yield of nosiheptide, hemoprotein from Sinorhizobium meliloti (SmHb) was overexpressed in S. actuosus with overexpression of hemoglobin from Vitreoscilla (VHb) as a positive control. The successful expression of SmHb and VHb in S. actuosus was confirmed by SDS-PAGE and CO-difference spectra analysis. The results showed that S. actuosus recombinant strain with SmHb expression improved nosiheptide production by 138% compared with the wild strain (WT) under the low aeration condition, which was similar to S. actuosus mutant strain with VHb expression. Furthermore, two copies of SmHb gene were integrated in S. actuosus, which further increased the nosiheptide production by approximately 1.9-fold higher than original strain, and final concentration of nosiheptide was up to 2352 μg/mL. These results suggested that engineering of SmHb could be used as an efficient method for constructing a high nosiheptide-accumulating strain.

Keywords:hemoprotein; Nosiheptide; Sinorhizobium meliloti; Streptomyces actuosus

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