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Symbiotic Efficiency, Genetic Diversity and Phylogeny of the Rhizobia Isolated from Leucaena Leucocephala in Arid-hot River Valley Area in Panxi，Sichuan , China
Xu Kaiwei 1 #,Petri Penttinen 2,Chen Yuanxue 3,Chen Qiang 3,Zhang Xiaoping 1 *
1.College of Resources and Environment, Sichuan Agricultural University
2.Department of Applied Chemistry and Microbiology, University of Helsinki, Helsinki, Finland
3.College of Resources and Environment, Sichuan Agricultural University
*Correspondence author
#Submitted by
Subject:
Funding: National Natural Science Foundation of China （No.No.31070004）, Specialized Research Found for the Doctoral Program of Higher Education of China （No.No.20060626006）, Educational Commission of Sichuan Province of China （No.No.09ZB047）
Opened online:28 March 2011
Accepted by: none
Citation: Xu Kaiwei,Petri Penttinen,Chen Yuanxue.Symbiotic Efficiency, Genetic Diversity and Phylogeny of the Rhizobia Isolated from Leucaena Leucocephala in Arid-hot River Valley Area in Panxi，Sichuan , China[OL]. [28 March 2011] http://en.paper.edu.cn/en_releasepaper/content/4415719
 
 
The symbiotic efficiency and diversity of the forty-two indigenous rhizobial isolates obtained from the root nodules of Leucaena leucocephala, one of the pioneer leguminous tree species of planted forests in arid-hot river valley area in Panxi, Sichuan, China, were characterized. The result of the inoculation test on their original host plants showed that the majority (86%) of the tested strains were ineffective. The genetic diversity of these rhizobia and the 17 reference strains were investigated by using PCR-RFLP of the 16S rRNA gene, 23S rRNA gene and the internal transcribed spacer (IGS) region between the 16S rRNA and 23S rRNA genes. The RFLP analyses and phylogenetic analyses based on the16S rRNA gene sequences of representative strains showed that Leucaena leucocephala hosted strains from four rhizobial genera, Sinorhizobium, Bradyrhizobium, Rhizobium and Mesorhizobium. One of the nodulating strains did not cluster with any of the reference strains. Phylogenetic analysis of 16S rRNA gene sequence of the strain indicated that the closest related genus was Herbaspirillum within the β-proteobacteria. This is the first record that Herbaspirillum of β-proteobacteria can be a specific symbiont of leguminous plant.
Keywords:Leucaena leucocephala; Rhizobia; symbiotic efficiency; phylogeny; Arid-hot River Valley
 
 
 

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