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Identification, genotyping, and molecular evolution analysis of duck circovirus
ZHANG Zhilong 1,JIA Renyong 2 * #,LU Yanyan 2,XU Yu 2,WANG Mingshu 3,CHENG Anchun 3
1.Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130
2.Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China
3.Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Ya'an, 625014, China
*Correspondence author
#Submitted by
Subject:
Funding: Sichuan Province Research Program（No.2009JY0070/11ZA084）, Research Fund for the Doctoral Program of Ministry Education （No.20125103110013）
Opened online: 2 July 2013
Accepted by: none
Citation: ZHANG Zhilong,JIA Renyong,LU Yanyan.Identification, genotyping, and molecular evolution analysis of duck circovirus[OL]. [ 2 July 2013] http://en.paper.edu.cn/en_releasepaper/content/4549443
 
 
Duck circovirus (DuCV) is a contagious immunosuppressive virus affecting many duck species, which is responsible for multiple outbreaks in poultry industries worldwide. In this study, the first DuCV isolate GH01 was identified in Sichuan by PCR, which shared a high level of nucleotide identity (81.8-99.4%) with sequences of other DuCV isolates available in GenBank. Comparative phylogenetic and pairwise sequence comparisons analyses indicated that DuCV could be divided into two genotypes (DuCV-1 and DuCV-2) and six subtypes (1a, 1b, 1c, 2a, 2b and 2c) based on the complete genome sequence. The results revealed that both DuCV-1 and DuCV-2 had evolved from the same ancestor but undergone divergent evolution. Interestingly, phylogenetic analyses indicated three isolates were classified into a cluster using complete DuCV genome sequence and cap gene, except rep gene. Recombination analyses revealed that DuCV-2a arose from recombination between DuCV-1a and DuCV-2c isolates within the rep genes, and the recombination events mainly occur both in non-structural protein coding region and structural protein coding region. In addition, the mechanisms of recombination supporting the genetic variability in DuCV isolates were investigated. Likewise, selective pressure indicated purifying selection had been a major driving force in maintaining diversity among the DuCV isolates. Because eradicating the virus from commercial ducks is impossible, it is necessary to take effective control measures and implement them throughout the world.
Keywords:duck circovirus; phylogenetic; genotype; evolution; recombination; selective pressure
 
 
 

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