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Development of Mutation Sensitive Switch Assays for Preeclampsia-associated AGT Polymorphism
LIU jinying 1,XING,Chungen 2,LI Kai 3 *
1.Laboratory of Molecular Medicine, College of Pharmaceutical Science, Soochow University, Suzhou 215123, China
2. Laboratory of Molecular Medicine, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China
3. Laboratory of Molecular Medicine, College of Pharmaceutical Science, Soochow University, Suzhou 215123, China
*Correspondence author
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Funding: Chinese National 863 major grant(No.NO.2008AA02Z436)
Opened online: 5 April 2016
Accepted by: none
Citation: LIU jinying,XING,Chungen,LI Kai.Development of Mutation Sensitive Switch Assays for Preeclampsia-associated AGT Polymorphism[OL]. [ 5 April 2016] http://en.paper.edu.cn/en_releasepaper/content/4682189
 
 
AGT (angiotensinogen) gene polymorphisms are associated with the development of hypertension and preeclampsia. Two main polymorphisms of AGT gene are T174M and M235T. The purpose of this study is to develop assays for detecting T174M and M235T by a nanoscale mutation-sensitive switch consisting of high fidelity polymerase and phosphorothioate-modified allele specific primers in order to meet the technical requirement in application for large scale genetic screening and for non-invasive genotyping of maternal circulating DNA. The developed assays showed sensitivities of as low as 10 copies and specificities of more than three log scales for matched over mismatched templates by routine PCR and real-time PCR. These assays are rapid, accurate, and cost-efficient in genotyping AGT gene for large population for the purpose of genetic screening.
Keywords:AGT; Polymorphism; Mutation-Sensitive On/Off Switch
 
 
 

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