| |
| To better understand the role of selenium (Se) in immunity, a few studies have been explored in some immune cells in vitro, but the effects of different forms of selenium on primary cultured pig splenocytes remain largely unclear. In present study, primary cultured pig splenocytes were incubated with 0 (control) and vary concentrations of selenium (Se) as selenomethionine (SeMet), or sodium selenite (Na2SeO3) for 48 h in the presence of ConA. Compared to controls, significantly higher T-cell proliferation were observed in groups treated by 0.5-32 μM SeMet, while T-cell proliferation were significantly increased in Na2SeO3-treated splenocytes at 0.5-4 μM, with maximal effects at 2 μM, and inhibited in a dose-dependent manner at 8-32 μM Na2SeO3. Intracellular reduced glutathione (GSH) contents and GPx activity in all Se-treated splenocytes were significantly higher than those in controls. An increases of GPx1 mRNA level were obtained in all Se-treated splenocytes, but GPx4 mRNA level was not affected by Se treatment. And mercaptosuccinic acid, the inhibitor of the GPx1, significantly inhibited the proliferation improved by Se. We conclude that regulations of ConA-induced T-cell proliferation by different Se forms are different in primary cultured pig splenocytes, and GPx1 maybe the key enzyme. Our finding may contribute to understanding the differential influences of Se in different forms on various types of immune responses. |
|
| Keywords:selenium; GPx1; T-cell proliferation; pig |
|
