Study on Endophytic fungi producing Orange Pigment isolated from Ginkgo Biloba L.

Jia-Jia Liu; Shu-Juan Chen; Han-Xiang Gong

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Study on Endophytic fungi producing Orange Pigment isolated from Ginkgo Biloba L.

Jia-Jia Liu 1 * #,Shu-Juan Chen 1,Han-Xiang Gong 2

1.Department of Pharmacy Engineering, college of chemistry and chemical engineering, central south university , Changsha, PR China

2.Suzhou Changshu bureau of Quality and Technique Supervision, Changshu, PR China

*Correspondence author

#Submitted by

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Funding: none

Opened online: 7 August 2008

Accepted by: none

Citation: Jia-Jia Liu,Shu-Juan Chen,Han-Xiang Gong.Study on Endophytic fungi producing Orange Pigment isolated from Ginkgo Biloba L.[OL]. [ 7 August 2008] http://en.paper.edu.cn/en_releasepaper/content/23292

More than seventy endophytic fungi were isolated by aseptic techniques from the phloem of the root of Ginkgo biloba L.. They were cultured in the potato dextrose (PD) liquid medium and one endophytic fungus (Gh01) was proved can produce orange pigment. The orange pigment was identified as quercetin glycoside by chemical reaction and HPLC. This is the first report on quercetin glycoside produced by endophytic fungus. The effects of the carbon and nitrogen sources, metal ions, initial pH and incubation temperature on pigment production of the endophytic fungus were evaluated. The optimal temperature and initial pH for pigment production in PD liquid medium were 28℃ and 7.0, respectively. Through the orthogonal trial, 20 g/L Glucose and 5 g/L peptone were the most suitable carbon and nitrogen source, 1 g/L chloridize zinc could increase the yield of pigment. Under the optimal conditions established, the maximum yield of the pigment was 27.515 g/L after 120 hours’ successive culture.

Keywords:Ginkgo biloba L; Endophytic fungi; Pigment; Quercetin glycoside; Optimization

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