Endogenous High-mobility Group Box 1 is Involved in the Progress of Benign Prostatic Hyperplasia by Promoting the Proliferation of Prostate Epithelial and Stromal Smooth Muscle Cells

Nie Lihong; Zhao Ruining; Xu Xiaolin; Qin Kaiyue; Li Yajie

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Endogenous High-mobility Group Box 1 is Involved in the Progress of Benign Prostatic Hyperplasia by Promoting the Proliferation of Prostate Epithelial and Stromal Smooth Muscle Cells

Nie Lihong 1,Zhao Ruining 2 *,Xu Xiaolin 2,Qin Kaiyue 3,Li Yajie 4

1.The Department of Physiology, School of Basic Medical Sciences, Ningxia Medical University

2.The Department of Urology, General Hospital of NingXia Medical University, Yinchuan, Ningxia, 750004, P.R.China

3. Class of Biotechnology Major, Grade 2013, Ningxia Medical University, Yinchuan, 750004

4.Class 6, Grade 2013, Clinical Major, Ningxia Medical University, Yinchuan, 750004

*Correspondence author

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Funding: Ningxia Natural Science Foundation （No.NZ16141, NGY2016091, 2015-NW-034, XT201419）, National Natural Science Foundation of China （No.No. 81460148, 81370855)）

Opened online:20 February 2017

Accepted by: none

Citation: Nie Lihong,Zhao Ruining,Xu Xiaolin.Endogenous High-mobility Group Box 1 is Involved in the Progress of Benign Prostatic Hyperplasia by Promoting the Proliferation of Prostate Epithelial and Stromal Smooth Muscle Cells[OL]. [20 February 2017] http://en.paper.edu.cn/en_releasepaper/content/4718958

Background: Our previous studies have demonstrated that chronic prostatic inflammation is involved in the pathogenesis and progression of benign prostatic hyperplasia (BPH), although its mechanisms are still unclear. High mobility group box 1 protein (HMGB1) is an important late inflammatory cytokine, which plays an important role in the initiation and amplication of the inflammation reaction. Additionally, HMGB1 induces tissue reorganization by promoting cell proliferation and migration. The present experiments were performed to determine the role of endogenous HMGB1 in the progress of BPH. Methods: Clinical information and tissue samples were obtained from 60 BPH patients. HMGB1 expression and distribution were analysed by RT-PCR, Western blotting and immunohistochemistry. Pearson correlation was analyzed to assess relationships among HMGB1 protein levels and each clinical parameters of BPH patients. In addition, Western blotting and ELISA were used to assay the endogenous production of HMGB1 in human prostate epithelial cells. The CCK8 assay and transwell migration assay were performed to examine the proliferation and migration of human prostate epithelial and stromal smooth muscle cells. Results: Prostatic inflammation increased HMGB1 expression in BPH patients, and HMGB1 protein could be secreted into the cytoplasm from nucleus of prostate epithelial cells subjected to prostatic inflammation. HMGB1 protein levels was positively correlated with prostate volume of BPH patients. In addition, LPS increased HMGB1 expression in prostate epithelial cells and led HMGB1 to be secreted into the extracellular space by prostate epithelial cells. Furthermore, HMGB1 promoted the proliferation of prostate epithelial and stromal smooth muscle cells and the migration of prostate stromal smooth muscle cells. Conclusions: the present study indicated that HMGB1 is involved in the progress of BPH and may represent a potential strategy to treat BPH.

Keywords:benign prostatic hyperplasia; high mobility group box 1 protein; cytokines; inflammation

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